Figure 3
From: Role of the Wnt receptor Frizzled-1 in presynaptic differentiation and function
Expression of Fz1 in hippocampal neurons during development. (A) Immunoblots of protein extracts from cultured hippocampal neurons during differentiation from 4 to 14 DIV. The same amount of protein was applied to all lanes. Molecular weight standards are indicated at the right (kDa). N-Cad, N-cadherin. (B) Immunodetection of Fz1 and phosphorylated MAP1B in cultured hippocampal neurons at 4 DIV. (C-E) Immunodetection of Fz1 and Bassoon (scale bar = 10 μm). (C) On DIV 7, Fz1 is observed in isolated axons as fine puncta. (D) On DIV 10, larger clusters of Fz1 co-localize with Bassoon puncta in axons contacting other neurons (arrows), but not in isolated axons (E).