Figure 1

Comparison of endogenous ptf1a RNA expression and GFP reporter protein. Expression in wholemount transgenic zebrafish heads is shown at different ages (hours post-fertilization; times are identical in both panels). (A) Endogenous ptf1a RNA expression was revealed by in situ hybridization visualized by BM purple. (B) GFP reporter protein expression of Tg(ptf1a:GFP) embryos. Stable GFP expression follows endogenous RNA expression with a temporal delay of a few hours and is maintained. In contrast, endogenous RNA expression of ptf1a occurs relatively transiently and disappears from differentiated neural tissue by 65 hpf. At this age, it is only maintained in the ciliary margin of the eye, where neurons continue to develop throughout adulthood. Scale bar = 1 mm.