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Figure 8 | Neural Development

Figure 8

From: Dynamic Pax6 expression during the neurogenic cell cycle influences proliferation and cell fate choices of retinal progenitors

Figure 8

Effects of altering Pax6 expression on cell proliferation and fate specification. Chicken retinal explants were transfected with Pax6 shRNA expression or Pax6 overexpression vectors at E4.5 (HH stage 24). Cell marker assays were performed after 3 days in vitro. Statistically significant sample pairs are indicated with asterisks and specific P-values (N ≥ 4). (A-E) Bar graphs showing percentages of cell marker and green fluorescent protein (GFP) double positive cells over total GFP+ transfected cells. (F) Compilation of cell type markers among all transfected GFP+ cells under control and Pax6 perturbation conditions. The mean values were used to generate the graph. (G) Summary of the percentages of cell markers and deviations (N ≥ 4, mean ± standard error) under controls and Pax6 knockdown or Pax6 overexpression conditions. BrdU, bromodeoxyuridine; PCNA, proliferating cell nuclear antigen.

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Neural Development

ISSN: 1749-8104