Figure 6

EB1-GFP dynamics. (A-b") Time-lapse imaging of EB1-GFP dynamics revealed diffuse low-level fluorescence and bright punctate comets (arrows) distributed throughout the axons and dendrites of hippocampal neurons developing in vitro. Representative still frames from movies, with higher magnification views of the boxed areas, of neurons at 1 DIV (A) (dendrite (a') and axon (a")) and 15 DIV (B) (dendrite (b') and axon (b")). Scale bar: 15 μm (A, B). See also accompanying Additional files 2 (1 DIV dendrite), 3 (1 DIV axon shaft), 4 (15 DIV dendritic arbor), 5 (15 DIV axon midshaft), and 6 (15 DIV distal axon and growth cone, not shown in figure).