Figure 6

UNC-7 and UNC-9::GFP co-localize. (A) Section of ventral nerve cord (vnc) in a wild-type (N2) animal transformed with UNC-9::GFP. UNC-9::GFP signal enhanced with anti-GFP antibody (green); anti-UNC-7 (red); co-localization (yellow). UNC-9::GFP in body muscle seen in upper half of photo. (B) Co-localization in pharyngeal nerve ring (ph). (C) Co-localization (arrow) in an unc-7(e5) animal rescued for forward locomotion with unc-7S construct (Figure 3C, minus GFP). (D) UNC-9Δ::GFP expression in L1 stage, and (E) in head neurons of L4 stage.