Figure 2

unc-7 mutations. (A) The unc-7 locus showing the approximate sites of rearrangements within intron 1 resulting in an Unc-7 phenotype. mn382 is a translocation, mn383 is a 6-kb deletion, and mn384 is a 2-kb insertion. (B) unc-7 point mutations and their location in the largest UNC-7 isoform (UNC-7L, 522 amino acids). ATG at M121 is the conserved innexin initiation site and start site of UNC-7SR. The position of GFP in unc-7S::gfp is also indicated. Asterisks indicate stop codons. EL, extracellular loop; TM, transmembrane domain. (The cold sensitive unc-7(hs10) allele was originally used as the basis for defining the gene unc-124 [50], and a heteroallelic interaction with unc-7 was reported [21, 50]. Since hs10 is a mutant allele of unc-7, the existence of unc-124 is dubious. See Materials and methods for details.)