Table 1 Primary antibodies
From: Cyclin D1 fine-tunes the neurogenic output of embryonic retinal progenitor cells
Antigen | Host | Target (relevant to this study) | Dilution factor | Source |
|---|---|---|---|---|
BHLHB5 | Goat | amacrine precursors1 | 1000 | Santa Cruz (sc-6045) |
BrdU | Mouse | cells that have uptaken BrdU in S-phase | 100 | BD biosciences (clone B44) |
BrdU | Rat | cells that have uptaken BrdU in S-phase | 50-200 | Serotec (clone BU1/75) |
POU4F2 | Goat | RGC precursors | 50 | Santa Cruz (sc-6026) |
CASPASE-3 | Rabbit | dying cells | 500 | BD biosciences (clone C92-605) |
VSX2 | Sheep | RPCs | 400 | Exalpha Biologicals (X1180P) |
CCND1 | Rabbit | RPCs | 400 | Lab Vision (RB-212) |
CCND1 | Mouse | RPCs | 400 | Santa Cruz (clone 72-13G) |
ISL1 | Mouse | amacrine cells 1 and RGCs 1 | 50 | DSHB (clone 39.4D5) |
HES1 | Rabbit | RPCs | 800 | Nadean Brown |
MITF | Mouse | RPE | 500 | Exalpha Biologicals (clone C5) |
NEFM | Rabbit | RGC and horizontal cells | 1000 | Chemicon (AB1987) |
NR2E3 | Rabbit | rod precursors | 100 | Anand Swaroop |
OTX2 | Rabbit | photoreceptor and amacrine precursors1 | 1000 | Chemicon (AB9566) |
OTX2 | Goat | photoreceptor and amacrine precursors1 | 400 | Santa Cruz (sc-30659) |
PAX6 | Mouse | RPCs | 10 | DSHB (clone P3U1) |
PCNA | Mouse | RPCs | 500 | DAKO (clone PC10) |
PCNA | Rabbit | RPCs | 100 | Santa Cruz (sc-7907) |
pHH3 | Rabbit | mitotic cells | 500 | Upstate Biotechnology (06-570) |
PTF1A | Rabbit | amacrine1 and horizontal precursors | 800 | Helena Edlund |
RCVRN | Rabbit | photoreceptor cells | 4000 | Chemicon (AB5585) |
RXRγ | Rabbit | cone and RGC precursors | 200 | Santa Cruz (sc-555) |
SOX2 | Rabbit | RPCs and amacrine cells 1 | 400 | Abcam (ab15830) |
acTUBB3 | Rabbit | neuronal precursors | 4000 | Covance (PRB-435P) |
acTUBB3 | Mouse | neuronal precursors | 1000 | Covance (clone TUJ1) |