Figure 6

Vsx2/Vsx1 down-/upregulation, followed by Vsx2 upregulation. Time-lapse images from Additional file 3 of a double transgenic Tg(vsx1:GFP; vsx2:dsRed) embryo. Vsx2 is initially expressed in the majority of progenitors in the developing neuroepithelium and becomes downregulated in cells that upregulate Vsx1. These cells continue to undergo cell divisions at the apical surface (white arrows) and start differentiating around 40 hours post-fertilization (hpf). Later (around 55 hpf), a subpopulation of cells starts upregulating the expression of Vsx2:DsRed (red arrows) and then differentiates in the inner nuclear layer. Scale bar: 500 μm.