IgCAMs redundantly control axon navigation in Caenorhabditis elegans

Table 3 Motoneuron defects in IgCAM mutants

	DD/VD motoneurons				DA/DB motoneurons
Gene	Cell body	VC	Side	Navig.	Cell body	VC	Side	DA2/DB3	Navig.
Wild type
hdIs22	1	4	12	7	0	0	3	0	1
Single mutants
rig-1(hd15) X	4	4	12	9	0	3	1	0	3
rig-3(hd51) X	0	3	9	5	0	0	0	0	0
rig-4(hd47) IV	0	1	21	2	0	0	5	0	0
rig-5(hd48) I	0	2	6	15	0	1	0	0	0
rig-6(gk376) II	2	2	9	6	0	0	6	0	0
rig-6(ok1589) II	1	8	21	28*	0	1	6	0	0
syg-1(hd18) X	5	4	12	4	1	1	3	10*	1
wrk-1(hd45) X	27*	5	9	8	3	0	5	0	0
ncam-1(hd49) X	0	2	15	9	0	0	2	0	0
Multiple mutants
wrk-1 ncam-1 X	28*	14^†	17	15	1	0	8	0	0
syg-1 wrk-1 X	26*	11	29*	19^†	0	0	7	13*	0
syg-1 ncam-1 X	1	7	23^†	5	0	0	5	12*	0
*syg-1 wrk-1 ncam-1* X	34*	4	18	12	3	0	6	18*	0
rig-6 II; rig-4 IV; syg-1 X	1	4	18	3	0	0	2	7*	0
rig-6 II; rig-4 IV; wrk-1 X	21*	3	15	6	3	0	12^†	2	0
rig-4 IV; syg-1 wrk-1 X	24*	6	8	4	2	1	6	12*	0
rig-6 II; syg-1 wrk-1 X	19*	9	10	3	1	1	5	9*	0
rig-6 II; rig-4 IV; syg-1 wrk-1 X	35*	7	21	8	4^†	4^†	5	14*	0
rig-6 II; rig-4 IV; *syg-1 wrk-1 ncam-1* X	22*	7	24^†	4	2	0	4	22*	0
rig-5 I; rig-6 II; rig-1 rig-3 X	0	1	13	8	0	6^†	7	0	1
rig-5 I; rig-6 II; rig-4 IV; rig-1 *wrk-1* rig-3 *syg-1 ncam-1* X	29*	13^†	43*	20*	1	2	9	21*	0

Alleles used were: syg-1 (hd18); wrk-1 (hd45); ncam-1 (hd49); rig-1 (hd15); rig-3 (hd51); rig-4 (hd47); rig-5(hd48); rig-6(gk376). Cell body: cell body outside ventral cord. VC: ventral cord crossover/outgrowth defects. Side: commissure growing on wrong side (DA2/DB3 excluded). DA2/DB3: commissures of those two motoneurons growing on wrong side. Navig.: commissure not reaching dorsal cord. n = 100, numbers in table are percent of animals with defects. *Significant with p < 0.01; ^†significant with p < 0.05; χ² test.

ISSN: 1749-8104