Figure 5

gbx1 morpholinos affect hindbrain patterning. (A-C') Embryos at the four somite stage stained for otx2/krox20/myod. (A) Control embryo, (B, B') embryos injected with 10 ng Mo1, (C, C') embryos injected with 7 ng Mo2. Injections of two different morpholinos show the same phenotype morphologically and after in situ hybridization analysis. (D-F) Rescue experiments using the krox20 expression domain as a read-out. (D) gbx1-morphant, (E, E') rescued morphant embryos co-injected with gbx1 mRNA that does not contain the MO binding sequence in its 5' region. (F) Two combinations were tested: 10 ng of Mo1 with 200 pg or 400 pg of gbx1 mRNA. (G, H) Western blot detection showing down regulation of gbx1-myc mRNA translation in embryos co-injected with gbx1-myc mRNA containing morpholino binding sequences and Mo1 (G) or Mo2 (H). WT, wild type.