Figure 9
From: Axonin-1/TAG-1 is required for pathfinding of granule cell axons in the developing cerebellum
AX-1 is not required for neurite extension on laminin. (a-i) Granule cells were plated on laminin in the absence of antibodies (AB) (a-c), in the presence of non-immune antibodies (d-f), and in the presence of goat anti-AX-1 antibodies (g-i). Neurites were stained with a rabbit anti-neurofilament (NF) antibody (a,d,g). Granule cells were identified by Pax6 (b,e,h). Merged images are shown in (c,f,i). Neurite morphologies and lengths did not differ under these conditions. Values were 101.0 ± 7.3 μm (n = 27) in the absence of antibodies, 101.1 ± 9.7 μm (n = 23) in the presence of non-immune, and 94.5 ± 7.6 μm (n = 31) in the presence of anti-AX-1 antibodies. Bar: 25 μm.