Figure 7

The canonical Wnt signaling pathway increases presynaptic inputs. (A) Representative images of Microtubule associated protein 2 (MAP2; dark blue) and Vesicular glutamate transporter 1 (VGLUT1; green) staining at 14 days in vitro (14 DIV) after 48 hours of treatment with NaCl (Control; left; 4 mM) or LiCl (right; 4 mM). Scale bar = 2 μm. (B) Quantification of the number of VGLUT1 puncta per area of dendrite (± standard error of the mean). LiCl treatment was normalized to the control condition (n = 58 field of views; **p < 0.001, Students t-test) (C) Representative images of dendrites immunostained with green fluorescent protein (GFP; dark blue) and VGLUT1 (green) at 14 DIV after transfection of GFP (left), constitutively active (CA) β-catenin (middle) or axin (right) at 7 DIV. Scale bar = 2 μm. (D) Bar histogram showing the quantification of the number of VGLUT1 puncta per area of dendrite (± standard error of the mean). All conditions were normalized to the control condition (n = 134 cells; *p < 0.05, **p = 0.001, ANOVA, then Tukey test). (E) Diagrammatic representation of the effects of Wnt, LiCl, and Axin on the regulation of β-catenin