| |
Phenotype (%)
|
|---|
|
Genotype
|
n
|
Two
|
One/none
|
Cross-over
|
|---|
|
Ptp4E
1
|
128
|
2
|
0
|
0
|
|
Ptp10D
1
|
128
|
1
|
0
|
0
|
|
Ptp4E1 Ptp10D1; UAS-4E-GFP/elav
|
124
|
7*
|
0
|
0
|
|
Ptp4E
1
Ptp10D
1
|
126
|
30†
|
0
|
1
|
|
Ptp4E1; Ptp69D1/Df(3L)8ex25
|
80
|
3
|
0
|
0
|
|
Ptp10D1; Ptp69D1/Df(3L)8ex25
|
128
|
63
|
21
|
100
|
|
Ptp4E1 Ptp10D1; Ptp69D1/Df(3L)8ex25
|
106
|
54
|
16
|
100
|
- CNS longitudinal axons were scored by counting the number of longitudinal tracts in each hemisegment at the midpoint between commissures (visualized by residual 1D4 staining at stage 17). In wild-type embryos, there are three fascicles per hemisegment. n, total number of hemisegments (T2–A6) scored at stage 17. Two: hemisegments with only two longitudinal tracts where the outer bundle is missing or is fused to the medial fascicle. One/none: hemisegments with only one or no longitudinal tracts. Cross-over: segments where axons abnormally cross the midline. *Expression of UAS-4E-GFP in neurons using the elav-GAL4 driver produces incomplete rescue (from 30% to 7%, versus 1–2% in single mutants). However, the statistical difference between Ptp4E1 Ptp10D1 and Ptp4E1 Ptp10D1; UAS-4E-GFP/elav was highly significant (p < 0.0001, Chi-square test), indicating that pan-neural expression of Ptp4E rescues. † Statistical differences between Ptp4E1 Ptp10D1 and each of the single mutants were also significant (p < 0.0001, Chi-square test).
