Figure 5

Comparison of cerebellofugal (CF) axon trajectories with Rig-1 immunoreactive axons. (A) A schematic view showing DiD (1,1'-dioctadecyl-3,3,3',3'-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt)-labelled CF axons (green) and the relative positions of the parasagittal sections shown in (B-G) (indicated by red lines and the letters beneath). (B-G) DiD-labelled CF axons (green) and Rig-1 immunoreactivity (purple) in parasagittal sections of embryonic day 16 preparations. (C,E,G) Magnifications of (B,D,F), respectively. Parasagittal sections were located at a distance of 150 μm (B,C) from the floor plate on the ipsilateral side, and 100 μm (D,E) and 250 μm (F,G) on the contralateral side relative to the DiD injection site. Scale bar = 100 μm for (B,D,F); 50 μm for (C,E,G).