Expressing exogenous functional odorant receptors in cultured olfactory sensory neurons

Table 1 Primer sequences for this study

Primer name	Sequence
Eco OMP	GAATTCATGGCAGAGGACGGGCCACAGAAG
OMP Xho	CTCGAGTCAGAGCTGGTTAAACACCACAG
AC3F	CAGCCAGCAGCCAACATGCCG
AC3R	CTTCCAGCTCATCCTGCTGCTG
GolfF2	ATGGGCCTATGCTACAGCCTGCGG
GolfR2	CTGACCGGAACTGGTTCTCAGGGTTG
CNGF2	GGTGCTGGATTACTTCTCAGACAC
CNGR	CCAGGTAGCCATATTCAGGGTCAG
GAPDH-F	ATGGTGAAGGTCGGTGTGAACG
GAPDH-R	AGTGATGGCATGGACTGTGGTC
M72-F	GTTAACATGGCTGCAGAGAATCAATC
M72-R	GAATTCAAAGACTCTTCTCCTCAGTG
P2-F	GGATCCATGACCTGGGGAAACTGGAC
P2-R	GAATTCTAGTTTCTGAGGGCCCAGAG
I7-F	GTTAACATGGAGCGAAGGAACCACAC
I7-R	GAATTCACCATCTCTGCTGGATTTC
OR118F	ATGGCGAACAGCACTACTGTTACTG
OR118R	TGTCTGGCTGAACTTTTGGAACTTGC
OR203F	ATGGAGGTGAACAGGACCCTGGTGACT
OR203R	TGGCTTCATGATTTTTCTCAGAGCC
GFP-LTF	GAATTCGCCACCATGGTGAGCAAGGGCG
GFP-LTR	CAATTGCTTAAGATACATTGATGAGTTTGG
GFP-MH2	AATGAATGCAATAGTTGTTGATAACTTGTTTATTG
GFP-MH2R	CAATAAACAAGTTATCAACAACTATTGCATTCATT
mCRY-R	GCGGCCGCTTTACTTGTACAGCTCGTCCATGCC
NeuroD-F	CAAGGTGGTACCTTGCTACTCCAAG
NeuroD-R	GGAATAGTGAAACTGACGTGCCT
Mash-F1	TGCCAGGCTCTCCTGGGAATGG
Mash-R1	CTGGTTCGGATAGATACAAATAG

In the RT-PCR experiments, olfactory marker protein transcripts were amplified by primers Eco OMP and OMP Xho, Adenylyl cyclase 3 by AC3F and AC3R, G_αolf by GolfF2 and GolfR2, cyclic nucleotide gated channel A2 subunit by CNGF2 and CNGR, NeuroD by NeuroD-F and NeuroD-R, Mash1 by Mash-F1 and Mash-R1, GAPDH by GAPDH-F and GAPDH-R, receptor M72 by M72-F and M72-R, I7 receptor by I7-F and I7-R, P2 receptor by P2-F and P2-R, MOR118 by OR118F and OR118R, and MOR182-5 by OR203F and OR203R.

ISSN: 1749-8104