Figure 4
From: Expressing exogenous functional odorant receptors in cultured olfactory sensory neurons
Lentiviral vector mediated gene transfer in primary olfactory sensory neurons. A) When 105pfu/ml recombinant enhanced green fluorescent protein (EGFP)-expressing lentiviral vectors were added to the primary olfactory sensory neuron culture, all β-tubulin III (β-Tub III) positive neurons (red) expressed EGFP (green). B) Lentiviral infection and ectopic expression of EGFP do not alter olfactory sensory neuron (OSN) morphology or axon length. When normalized as 1 ± 0.19 in the control (n = 74), OSN axon average lengths were 1.02 ± 0.15 (n = 56; t-test p > 0.8) in GFP lentiviral vector-infected cultures and 1.00 ± 0.17 (n = 60; t-test p > 0.9) in mouse I7 lentiviral vector-infected cultures. Values are mean ± standard error from three independent experiments. Bar = 30 μm.