Figure 3
From: Expressing exogenous functional odorant receptors in cultured olfactory sensory neurons
Primary olfactory sensory neurons express endogenous odorant receptors. A) Odorant receptor transcripts were detected in cultured olfactory sensory neurons by RT-PCR. Cyclic nucleotide gated channel Ay subunit (CNGA2) primers were designed to discriminate PCR products amplified from genomic DNA (710 bp; G) or olfactory sensory neuron culture cDNA (429 bp; O). The odorant receptor transcripts were not detected in cortical astrocytes (As). All selected odorant receptor transcripts, including M72, P2, I7, MOR118 and MOR182-5, were detected in cultured olfactory sensory neurons at 3 days in vitro (DIV). B) Odorant receptor protein expression was detected in cultured olfactory sensory neurons. Olfactory sensory neurons were identified by β-tubulin III expression (green in ii). MOR42-3 immunostaining positive neurons (red in i) were detected among cultured olfactory sensory neurons (merge in iii) at 3 DIV. Bar = 20 μm.