Figure 1
From: Expressing exogenous functional odorant receptors in cultured olfactory sensory neurons
Primary olfactory sensory neuron culture. A) Olfactory sensory neurons were identified by β-tubulin III expression and were bipolar with a short dendrite-like and a long axon-like process, resembling their in vivo morphology. B) Percentage of bipolar neurons among all neuronal cells was at 41.1% after one day in vitro (DIV). The percentage of bipolar neurons increased to 99.7% at 6DIV. C) The average survival rate of the cultured olfactory sensory neurons, when compared to plating cell density, was 41.6 ± 4.2% at 1DIV, 29.9 ± 3.1% at 2DIV, 24.7 ± 2.7% at 3DIV, 19.3 ± 2.1% at 4DIV and 16.9 ± 1.8% at 5DIV. Values are mean ± standard deviation from three independent experiments at each data point. Bar = 30 μm.