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Table 1 Postnatal retinal cell genesis as quantified by thymidine birthdating and either immunofluorescent or histological identification of cell fate

From: Temporal order of bipolar cell genesis in the neural retina

 

P0

P2

P4

P6

Cell type

IF

Hist*

IF

Hist*

IF

Hist*

IF

Hist*

Bipolar

2.19 ± 1.45

6.51

3.98 ± 2.66

17.04

12.81 ± 6.16

31.81

16.01 ± 6.31

25.74

   Cone type

1.67 ± 1.15

NA

2.73 ± 1.90

NA

7.96 ± 3.23

NA

8.20 ± 2.97

NA

   Rod type

0.52 ± 0.32

NA

1.21 ± 1.01

NA

4.85 ± 3.35

NA

7.82 ± 3.41

NA

Amacrine

NA

9.16

NA

0.05

NA

0.00

NA

0.00

Rod

69.69 ± 12.65

81.67

85.73 ± 4.15

79.51

71.22 ± 5.88

59.54

62.74 ± 8.63

60.59

Muller

0.68 ± 0.26

2.66

0.58 ± 0.43

3.40

2.73 ± 1.08

8.65

4.04 ± 1.97

13.67

  1. Postnatal mice were injected with tritiated thymidine at the developmental time indicated. For the immunofluorescent (IF) methodology, retinae were harvested at P16, dissociated and stained using established cell-type specific markers, namely anti-Chx10 (bipolar cells), Rho4D2 (rod photoreceptors), and anti-glutamine synthetase (Muller cells). *For the histochemical (Hist) estimates all data are based on [19]. Cells born from the central and peripheral sections were summed to thereafter derive a percentage of each cell type