Figure 4

Immunohistochemical staining of rod and cone bipolars in the mouse retina in tissue section. Rod bipolars are double stained with Chx10 and PKC antibodies, while cone bipolars are positive for Chx10 staining in the nuclei but are not stained by PKC antibodies. Confocal images of P21 mouse retinal sections stained with Chx10 antibody (green nuclear stain) and PKC antibodies (red staining throughout bipolar processes). The margins of the retina are indicated with dashed lines. Rod bipolars are double labeled (yellow nuclei and red processes that extend into the inner half of the IPL and characteristic dendritic process). Cone bipolars are labeled by only Chx antibodies (green nuclei in the INL). GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; OPL, outer plexiform layer.