Figure 3
HOP regulates apoptosis of SGL astrocytic stem cells. (a) Apoptotic cells are detected in the SGL (arrows) with a TUNEL fluorescent assay. These cells are relatively rare (asterisks). Scale bar: 50 ÎĽm. (b) The number of TUNEL positive apoptotic cells in the SGL was counted across the entire DG (36 slices per mouse). A significant decrease of 48.2% is observed in knock out (KO) mice compared to wild type (WT) (n = 5, Mann-Whitney U test, *p = 0.001). (c-h) Double labeling for HOP and TUNEL shows that a majority of the TUNEL positive SGL cells are HOP immunoreactive (arrows). The arrowhead in (c-e) indicates a HOP negative TUNEL positive SGL cells (probably a D/type2 cell). The asterisk in (c-e) shows a TUNEL positive cell in the granule cell layer (probably a granular neuron). (i) Higher magnification of (h) with orthogonal projections along the z confocal stack at the x and y levels indicated by the intersecting white lines. Scale bars: 10 ÎĽm.