Figure 5
From: Polarity and intracellular compartmentalization of Drosophila neurons
Determination of microtubule polarity in axons and dendrites using EB1-GFP dynamics. Flies containing an elav-Gal4 transgene were crossed to flies with a UAS-EB1-GFP transgene. Progeny were aged to early L2 and whole larvae were mounted for imaging. Single confocal images were acquired every 3.6 seconds. Additional data file 1 is a movie that shows all images acquired. Frames from two portions of the movie were selected for still images. The top row shows EB1-GFP fluorescence in an axon of a dendritic arborization neuron. The arrow and arrowhead track two different EB1-GFP dots as they move away from the cell body. The bottom row shows the dendrite from the same neuron as the top panels; these images were acquired at a different plane of focus. Three different EB1-GFP dots are tracked. The one indicated with the upward-pointing arrow moves away from the cell body and the other two move towards the cell body. The figure is shown with anterior up and dorsal to the left. Scale bar is 5 μm.