Skip to main content

Table 1 Electroporation results at 48 hpf

From: Electroporation-based methods for in vivo, whole mount and primary culture analysis of zebrafish brain development

 

Transfection levels*

 

Voltage

+++

++

+

None

Dead

30 (n = 77†)

72 (94%)

3 (4%)

-

-

2 (3%)

20 (n = 16)

2

8

5

1

-

10 (n = 16)

-

-

5

11

-

  1. Injection of 0.7 mg/ml each pHuc:GAL4/pUAS:EGFP into the lateral forebrain and ventricle was followed by two manually applied trains of five 1 ms pulses at the indicated voltage. Transfection levels were greatly decreased with voltages lower than 30 V. *Embryos were viewed dorsally under a compound fluorescence microscope and categorized according to extent of transgene expression: +++, similar to Figure 2a or higher; ++, intermediate levels; +, few enough cells to make out and count individually, fewer than 20. †Of 80 embryos electroporated in 2 experiments, 3 were killed by mechanical damage during removal from agarose and are not included.
Back to article page

Neural Development

ISSN: 1749-8104