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Table 1 Transcripts differentially expressed in the DRG and TG

From: POU-domain factor Brn3a regulates both distinct and common programs of gene expression in the spinal and trigeminal sensory ganglia

Transcript Symbol Class Fold
Greater expression in the DRG    
   Homeobox B3 Hoxb32 TX (90.4)
   Homeobox A9 Hoxa9 TX (90.2)
   Homeobox A5 Hoxa52 TX (44.2)
   Etv1; ER81 Etv13 TX (40.2)
   Homeobox C8 Hoxc82 TX (38.4)
   Homeobox B6 Hoxb6 TX (27.6)
   Hoxd4 Hoxd4 TX (16.6)
   Follistatin Fst2 Dev (12.9)
   Leucine-rich repeat-containing G protein-coupled receptor 6 Lgr6 Other (11.8)
   Receptor (calcitonin) activity modifying protein 2 Ramp23 ST 10.9
   Ca+ channel, L type, alpha 1C subunit Cacna1c NT 9.2
   Homeobox B8 Hoxb8 TX (9.1)
   BTB and CNC homology 2 Bach2 TX (8.8)
   Sclerostin domain containing 1 Sostdc1 Dev 8.5
   Laminin, alpha 2 Lama2 Other (7.3)
   Purkinje cell protein 4 Pcp4 Unk (5.7)
   LIM domain binding 2 Ldb22 TX 5.6
   Zinc finger protein of the cerebellum 1 Zic12 TX 5.3
   Gap junction membrane channel protein alpha 1 Gja12 NT 5.1
   Protein tyrosine phosphatase, receptor type, E Ptpre3 ST 5.0
   Early B-cell factor 1; Olf1 Ebf1 TX 5.0
   U74 array    
   Regulator of G-protein signaling 4 RGS4 NT 6.1
   Endothelin receptor type B EDNRB Other 3.0
   Anthrax toxin receptor 2 Antxr2 Unk 3.0
Greater expression in the TG    
   Microfibrillar-associated protein 4 Mfap4 Unk (20.6)
   Lectin, galactose binding, soluble 7 Lgals7 AX (11.3)
   MyoD family inhibitor Mdfi TX (9.6)
   Suppressor of cytokine signaling 3 Socs32 ST (6.4)
   RNA imprinted and accumulated in nucleus Rian Unk 6.1
   Musashi homolog 2 Msi2h Other 6.0
   U74 array    
   Neuropeptide Y receptor 1 NpyR1 NT 8.6
  1. The principal analysis was conducted with the murine 430 array, with selected results identified using the U74A+B array set added. All listed transcripts showed increased expression (change p < 0.005) in two independent replicates. Fold values represent the ratio of the means of two determinations for each tissue. Fold changes in parentheses indicate an absent call, that is, below the statistically reliable limit of detection, in the lower expressing sample. Transcripts with expression levels less than 40% of the scaled mean are excluded. Superscript numerals represent the number of probe sets concordant for changed expression for a given gene. AX, axonogenesis; Dev, development; NT, neurotransmission; ST, signal transduction; Syn, synaptogenesis; TX, transcription; Unk, unknown. Greater expression in the DRG: confirmation of selected results by in situ hybridization and immunofluorescence appears in additional file 1. Differential expression of Ramp2 could not be confirmed by ISH. Different levels of Zic1 and follistatin appear to be due to high expression in the spinal cord adjacent to the DRG, rather than the DRG itself. Sostdc1 and EDNRB showed increased DRG expression by ISH but in a pattern more characteristic of glial than neuronal expression, with signal concentrated in nerve roots. The expression of RGS4 in the developing sensory ganglia has been previously described [43], and its higher expression in the DRG at E13.5 appears to be due to the dynamic developmental regulation of this gene, rather than a persisting difference in expression between the DRG and TG. Greater expression in the TG: in situ hybridization confirmed some changes (see additional file 1). However, Mfap4 was widely expressed in embryonic tissues. Socs3 ISH exhibited relatively greater expression in the TG, but overall expression levels were low. Differential expression of the supressor of cytokine signaling Socs3 in the TG and DRG has previously been noted [44].