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Table 1 Transcripts differentially expressed in the DRG and TG

From: POU-domain factor Brn3a regulates both distinct and common programs of gene expression in the spinal and trigeminal sensory ganglia

Transcript

Symbol

Class

Fold

Greater expression in the DRG

   

   Homeobox B3

Hoxb32

TX

(90.4)

   Homeobox A9

Hoxa9

TX

(90.2)

   Homeobox A5

Hoxa52

TX

(44.2)

   Etv1; ER81

Etv13

TX

(40.2)

   Homeobox C8

Hoxc82

TX

(38.4)

   Homeobox B6

Hoxb6

TX

(27.6)

   Hoxd4

Hoxd4

TX

(16.6)

   Follistatin

Fst2

Dev

(12.9)

   Leucine-rich repeat-containing G protein-coupled receptor 6

Lgr6

Other

(11.8)

   Receptor (calcitonin) activity modifying protein 2

Ramp23

ST

10.9

   Ca+ channel, L type, alpha 1C subunit

Cacna1c

NT

9.2

   Homeobox B8

Hoxb8

TX

(9.1)

   BTB and CNC homology 2

Bach2

TX

(8.8)

   Sclerostin domain containing 1

Sostdc1

Dev

8.5

   Laminin, alpha 2

Lama2

Other

(7.3)

   Selected

   

   Purkinje cell protein 4

Pcp4

Unk

(5.7)

   LIM domain binding 2

Ldb22

TX

5.6

   Zinc finger protein of the cerebellum 1

Zic12

TX

5.3

   Gap junction membrane channel protein alpha 1

Gja12

NT

5.1

   Protein tyrosine phosphatase, receptor type, E

Ptpre3

ST

5.0

   Early B-cell factor 1; Olf1

Ebf1

TX

5.0

   U74 array

   

   Regulator of G-protein signaling 4

RGS4

NT

6.1

   Endothelin receptor type B

EDNRB

Other

3.0

   Anthrax toxin receptor 2

Antxr2

Unk

3.0

Greater expression in the TG

   

   Microfibrillar-associated protein 4

Mfap4

Unk

(20.6)

   Lectin, galactose binding, soluble 7

Lgals7

AX

(11.3)

   MyoD family inhibitor

Mdfi

TX

(9.6)

   Suppressor of cytokine signaling 3

Socs32

ST

(6.4)

   RNA imprinted and accumulated in nucleus

Rian

Unk

6.1

   Musashi homolog 2

Msi2h

Other

6.0

   U74 array

   

   Neuropeptide Y receptor 1

NpyR1

NT

8.6

  1. The principal analysis was conducted with the murine 430 array, with selected results identified using the U74A+B array set added. All listed transcripts showed increased expression (change p < 0.005) in two independent replicates. Fold values represent the ratio of the means of two determinations for each tissue. Fold changes in parentheses indicate an absent call, that is, below the statistically reliable limit of detection, in the lower expressing sample. Transcripts with expression levels less than 40% of the scaled mean are excluded. Superscript numerals represent the number of probe sets concordant for changed expression for a given gene. AX, axonogenesis; Dev, development; NT, neurotransmission; ST, signal transduction; Syn, synaptogenesis; TX, transcription; Unk, unknown. Greater expression in the DRG: confirmation of selected results by in situ hybridization and immunofluorescence appears in additional file 1. Differential expression of Ramp2 could not be confirmed by ISH. Different levels of Zic1 and follistatin appear to be due to high expression in the spinal cord adjacent to the DRG, rather than the DRG itself. Sostdc1 and EDNRB showed increased DRG expression by ISH but in a pattern more characteristic of glial than neuronal expression, with signal concentrated in nerve roots. The expression of RGS4 in the developing sensory ganglia has been previously described [43], and its higher expression in the DRG at E13.5 appears to be due to the dynamic developmental regulation of this gene, rather than a persisting difference in expression between the DRG and TG. Greater expression in the TG: in situ hybridization confirmed some changes (see additional file 1). However, Mfap4 was widely expressed in embryonic tissues. Socs3 ISH exhibited relatively greater expression in the TG, but overall expression levels were low. Differential expression of the supressor of cytokine signaling Socs3 in the TG and DRG has previously been noted [44].
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Neural Development

ISSN: 1749-8104