Figure 6

Over-expression of skp2 can degrade p27Xic1. (a) Western blot for embryos at stage 9, either uninjected or injected with 1 ng mRNA encoding wild-type (WT) skp2 or F box skp2, as labelled; tubulin demonstrates equal loading. (b) Western blot for Xic1 levels in embryos injected with 50 pg Xic1 mRNA and increasing doses (100 pg to 1 ng) of skp2 mRNA at the one cell stage. Embryos were harvested at stage 13; tubulin demonstrates equal loading. (c) Western blot for Xic1 levels in embryos harvested at stage 7 that were injected with 1 ng WT skp2 or 1 ng FBox skp2 mRNA at the one cell stage along with 50 pg Xic1 mRNA; tubulin demonstrates equal loading. (d) Western blot for Xic1 levels in neural plates harvested at stage 16 from embryos that were injected with 1 ng WT skp2 or 1 ng FBox skp2 mRNA into the animal pole of a fertilised egg. Tubulin demonstrates equal loading. (e-h) Embryos were injected with 1 ng WT skp2 (e,g) or 1 ng FBox skp2 (f,h) mRNA, along with ßgal mRNA as a lineage tracer, in one blastomere at the two cell stage. Embryos were analyzed for expression of muscle actin (e,f) or heavy chain myosin expression (g,h) at stage 15. Dorsal views with injected side to the right.