Over-expression of eve in aCC/RP2 is sufficient to alter electrical properties. (A) Over-expression of eve in aCC/RP2 results in a decrease in peak conductance of IKfast (78.6 ± 4.0 versus 62.3 ± 8.4 pA/pF, measured at +45 mV, p ≤ 0.05) and no significant change in INa (-23.4 ± 1.6 versus -21.4 ± 2.2 pA/pF, -15 mV), ICa (-15.7 ± 1.6 versus -17.1 ± 2.9 pA/pF, 0 mV) or IKslow (48.3 ± 3.5 versus 53.7 ± 6.8 pA/pF, +45 mV). (B) Example voltage clamp recordings (examples shown from wild-type aCC/RP2) of the four major voltage-gated currents in aCC/RP2; INa, ICa, IKfast (arrow shows current measured) and IKslow (for a more detailed discussion of these conductances see ). INa, ICa, IKfast were evoked using the protocol shown (v) while IKslow was evoked using the protocol shown in (vi). (C) IV plots for IKfast measured following over-expression of eve, with or without ChTx, show no obvious changes in activation kinetics. (D) Peak current amplitudes of IKfast (measured at +45 mV) show significant decreases following either over-expression of eve or addition of ChTx. Simultaneous over-expression of eve and addition of ChTx does not further decrease IKfast, indicating that expression of eve specifically down-regulates the slowpoke component of this conductance. For (c,d) n = 8, mean ± SE, *p ≤ 0.05.