Figure 1
Over-expression of eve in aCC/RP2 is sufficient to alter electrical properties. (A) Over-expression of eve in aCC/RP2 results in a decrease in peak conductance of IKfast (78.6 ± 4.0 versus 62.3 ± 8.4 pA/pF, measured at +45 mV, p ≤ 0.05) and no significant change in INa (-23.4 ± 1.6 versus -21.4 ± 2.2 pA/pF, -15 mV), ICa (-15.7 ± 1.6 versus -17.1 ± 2.9 pA/pF, 0 mV) or IKslow (48.3 ± 3.5 versus 53.7 ± 6.8 pA/pF, +45 mV). (B) Example voltage clamp recordings (examples shown from wild-type aCC/RP2) of the four major voltage-gated currents in aCC/RP2; INa, ICa, IKfast (arrow shows current measured) and IKslow (for a more detailed discussion of these conductances see [34]). INa, ICa, IKfast were evoked using the protocol shown (v) while IKslow was evoked using the protocol shown in (vi). (C) IV plots for IKfast measured following over-expression of eve, with or without ChTx, show no obvious changes in activation kinetics. (D) Peak current amplitudes of IKfast (measured at +45 mV) show significant decreases following either over-expression of eve or addition of ChTx. Simultaneous over-expression of eve and addition of ChTx does not further decrease IKfast, indicating that expression of eve specifically down-regulates the slowpoke component of this conductance. For (c,d) n = 8, mean ± SE, *p ≤ 0.05.