Molecular and behavioral profiling of Dbx1-derived neurons in the arcuate, lateral and ventromedial hypothalamic nuclei
© Sokolowski et al. 2016
Received: 12 November 2015
Accepted: 4 May 2016
Published: 21 May 2016
Neurons in the hypothalamus function to regulate the state of the animal during both learned and innate behaviors, and alterations in hypothalamic development may contribute to pathological conditions such as anxiety, depression or obesity. Despite many studies of hypothalamic development and function, the link between embryonic development and innate behaviors remains unexplored. Here, focusing on the embryonically expressed homeodomain-containing gene Developing Brain Homeobox 1 (Dbx1), we explored the relationship between embryonic lineage, post-natal neuronal identity and lineage-specific responses to innate cues. We found that Dbx1 is widely expressed across multiple developing hypothalamic subdomains. Using standard and inducible fate-mapping to trace the Dbx1-derived neurons, we identified their contribution to specific neuronal subtypes across hypothalamic nuclei and further mapped their activation patterns in response to a series of well-defined innate behaviors.
Dbx1-derived neurons occupy multiple postnatal hypothalamic nuclei including the lateral hypothalamus (LH), arcuate nucleus (Arc) and the ventral medial hypothalamus (VMH). Within these nuclei, Dbx1 + progenitors generate a large proportion of the Pmch-, Nesfatin-, Cart-, Hcrt-, Agrp- and ERα-expressing neuronal populations, and to a lesser extent the Pomc-, TH- and Aromatase-expressing populations. Inducible fate-mapping reveals distinct temporal windows for development of the Dbx1-derived LH and Arc populations, with Agrp+ and Cart+ populations in the Arc arising early (E7.5-E9.5), while Pmch+ and Hcrt+ populations in the LH derived from progenitors expressing Dbx1 later (E9.5-E11.5). Moreover, as revealed by c-Fos labeling, Dbx1-derived cells in male and female LH, Arc and VMH are responsive during mating and aggression. In contrast, Dbx1-lineage cells in the Arc and LH have a broader behavioral tuning, which includes responding to fasting and predator odor cues.
We define a novel fate map of the hypothalamus with respect to Dbx1 expression in hypothalamic progenitor zones. We demonstrate that in a temporally regulated manner, Dbx1-derived neurons contribute to molecularly distinct neuronal populations in the LH, Arc and VMH that have been implicated in a variety of hypothalamic-driven behaviors. Consistent with this, Dbx1-derived neurons in the LH, Arc and VMH are activated during stress and other innate behavioral responses, implicating their involvement in these diverse behaviors.
KeywordsDbx1 Hypothalamus Behavior Neuronal fate Neuronal development
The hypothalamus plays a critical role in a variety of behaviors essential for survival, species propagation and maintenance of homeostasis. These behaviors most prominently include regulation of hunger and body temperature states, mating, aggression and appropriate responses to threatening encounters [1–5]. Proper development of this system is critical for normal function and behavior, and altered trajectories of development may potentially contribute to diseases or disorders such as obesity, depression and anxiety [4, 6].
At the anatomical level, the hypothalamus is composed of multiple nuclei each characterized largely by their location in the rostral-caudal plane, molecularly and functionally distinct diversity of neuronal cell types and involvement in different aspects of behaviors. Different nuclei, as well as the constellation of neuronal subtypes, have specific and overlapping roles in coordinating complex animal behaviors. For example, the arcuate nucleus (Arc) is linked mostly to regulating hunger and satiety states [2, 7]. The primary role of the lateral hypothalamus (LH) is to create a state of arousal [8, 9], and as such is implicated in diverse behaviors such feeding/food seeking, mating, aggression and predator threat responses. Another major nucleus is the ventromedial hypothalamus (VMH), which appears to be involved in, if not critical for, aggressive and reproductive behaviors as well as innate fear responses [1, 10, 11].
These diverse behaviors appear to be regulated by different molecularly identifiable neuronal subpopulations located within these distinct nuclei [12–15]. Neuronal identity of mature hypothalamic neurons is presumably endowed during embryonic development through the combinatorial expression of both distinct and overlapping sets of transcription factors [16–18]. While the complete developmental blueprint for specification of different hypothalamic neuronal subpopulations remains to be elucidated, some of the key intrinsic and extrinsic factors controlling specification and differentiation have begun to be elucidated . In our recent studies, we revealed that the homeodomain-containing transcription factor encoding gene Dbx1 acts as a putative selector gene for the generation of the pro-melanin-concentrating hormone (Pmch)+, Calbindin+, and orexin/hypocretin (Hcrt)+ neurons in the LH and Agouti-related peptide (Agrp)/ Neuropeptide Y (Npy)+ neurons in the Arc . At the behavioral level, we further found that Dbx1 is required for hypothalamic-mediated feeding and stress responses in adult animals. However, despite this understanding of Dbx1 gene function, the contribution of Dbx1-derived neurons to different hypothalamic neuronal populations and activation of Dbx1-derived neurons during innate behaviors currently remains unknown.
To determine the contribution of the Dbx1-lineage to distinct neuronal subpopulations within LH, Arc and VMH, by using both standard and inducible fate-mapping techniques we significantly expand upon existing Dbx1 lineage studies conducted by the Allen Institute for Brain Science (Allen Brain Atlas experiment 167643944). We found that within these regions, Dbx1-derived neurons contribute to diverse populations of molecularly identified neuronal subpopulations. Moreover, we characterized the behavioral activation patterns of Dbx1-derived neurons in the LH, Arc and VMH after exposure to a broad array of innate behavioral cues. We found that Dbx1-derived neurons are activated by predator odor, fasting, mating and male aggression, without specific tuning toward one behavior. Therefore, across hypothalamic nuclei Dbx1-derived neurons likely play a critical role in regulation of different innate behaviors.
Mice were housed in the temperature- and light-controlled Children’s National Medical Center animal care facility and given food and water ad libitum, unless otherwise stated. All animal procedures were approved by Children’s National Medical Center’s Institutional Animal Care and Utilization Committee (IACUC) and conformed to NIH Guidelines for animal use.
ROSA loxP-STOP-loxP-YFP (RYFP) reporter mice were obtained from Jackson Labs (stock no: 006148). Dbx1 Cre mice were kindly provided by Dr. Alessandra Pierani . Dbx1 Cre ERT2 mice were previously generated in the Corbin lab  (available at Jackson Labs, stock no: 028131). For fate-mapping analysis Dbx1 Cre+/- ;RYFP +/- mice were obtained by crossing Dbx1 Cre+/- males with RYFP +/+ females. For genetically inducible fate-mapping analysis Dbx1 CreERT2+/-; RYFP +/- mice were obtained by crossing Dbx1 CreERT2+/-;males with RYFP +/+ females. Mice were genotyped by Transnetyx Inc. Genotyping Services (Cordova, TN). Noon on the day of vaginal plug was designated as E0.5. For inducible fate mapping, tamoxifen (Sigma T5648) was dissolved in sesame oil (Sigma S3547) to a concentration of 3 mg/ml. Pregnant females were given 100 μl (0.3 mg of tamoxifen; approximately 0.1 mg/grams body weight) via oral gavage with animal feeding syringes (FisherBrand 01-208-87).
Tissue processing, in situ hybridization (ISH) and immunohistochemisty (IHC)
For ISH, embryos were dissected and fixed in 4 % paraformaldehyde overnight and then cryoprotected in 30 % sucrose. Embryonic brains were sectioned on a cryostat at 20 μm. Every tenth section was collected in a set, for a total of ten sets of sections representing the entire brain. One probe was run on one set of sections; 10 probes were run on serial sections from the same brains. RNA in situ hybridization was performed as previously described . cDNA plasmids were obtained from Drs. Seth Blackshaw (Pmch and Hcrt), Paul Gray (Agrp and Pomc), Yasushi Nakagawa (Lhx9), Chen-Ming Fan (Sim1), Kenneth Campbell (Dbx1), and Kazue Hashimoto-Torii (Nr5a1 and Fezf1). Images were taken on an Olympus BX51 at 4x and 10x.
For IHC, postnatal day (P) 21 mice were perfused with 4 % paraformaldehyde. Postnatal brains were sectioned at 50 μm on a vibratome (Leica VT1000 S). Every sixth section was collected in one well, with six wells containing every section from the brain. Embryonic brains were processed as described above and used for IHC. Immunofluorescent staining on sections was performed as previously described . Primary antibodies used were: rat anti-GFP (1:1000, 04404-84, Nacalai, Kyoto Japan), goat anti-Pomc (1:100, ab322893, Abcam), goat anti-Pmch (1:1000, sc-14509, Santa Cruz), goat anti-Agrp (1:1000, AF634, R&D Systems), rabbit anti-c-Fos (1:1000, sc52, Santa Cruz), rabbit anti-Hcrt (1:1000, AB3096, Millipore), rabbit anti-TH (1:500, sc14007, Santa Cruz), rabbit anti-Cart (1:20,000, 55-102, Phoenix Pharmaceuticals), sheep anti-Nesfatin (1:500, AF6895, R&D Systems) and rabbit anti-Dbx1 (1:100) . Secondary antibodies were: donkey anti-rat IgG Alexa 488 (1:1000 Invitrogen), donkey anti-rabbit Cy3 or Cy5 (1:1000 Jackson ImmunoResearch), donkey anti-goat Cy3 or Cy5 (1:1000 Jackson ImmunoResearch) and donkey anti-sheep Cy3 or Cy5 (1:1000 Jackson ImmunoResearch). Co-labeling was determined after images were taken on an Olympus FV1000 confocal microscope at 20x or 40x magnification on an optical slice of 1–3 μm.
Analysis of double labeled IHC stained sections was performed on every sixth section of the brain containing the region of interest. LH, Arc and VMH were defined from Bregma -1.06 to -1.94, -1.22 to -2.06 and -1.34 to -1.70, respectively, using the Paxinos and Watson anatomical atlas. DAPI (DAPI Fluoromount-G, SouthernBiotech #0100-20) was used as a nuclear counterstain to aid the definition of hypothalamic nuclei. Quantification of c-Fos and co-labeled cells in LH, Arc and VMH were analyzed across 3, 2 and 1 sections, encompassing each nuclei, per animal, respectively. The average total number of YFP+ cells counted per animal was 1027, 546, and 1055 in the LH, Arc, and VMH, respectively. Cell counts were expressed as the number of neurons/mm2 for each animal. Animals within a group were then averaged.
Unless otherwise stated, all animals were group-housed by sex after weaning and then singly housed and habituated to the behavioral assay 1 week prior to experiment, which took place >1 h after the beginning of the dark cycle. Mating, aggression, predator avoidance and fasting assays were performed in adult mice (P40-50) as previously reported . Animals were sacrificed 1 h after the start of the behavioral paradigm and brains processed for c-Fos and YFP IHC.
Quantitation of data was performed blind to relevant variables. Using GraphPad Prism 6 statistical software, a One-way ANOVA followed by Tukey–Kramer multiple comparison test was used for analysis of experiments involving three groups with one comparison (Figs. 6 and 7, comparing cell counts at 3 different labeling periods: TME7.5, 9.5, and 11.5), and an unpaired t-test with Welch’s correction was used for analysis of experiments involving two groups.
Regional fate of Dbx1-derived neurons
Identity of Dbx1-derived neurons
Pmch-expressing neurons are one of the two major output populations of the LH and respond to high concentrations of blood glucose . Pmch+ neurons are defined by their co-expression with Nesfatin (Nfn)- and Cocaine and amphetamine regulated transcript (Cart) [34–36]. Our previous Dbx1 loss-of-function studies revealed that Pmch neurons are specified by Dbx1-dependent mechanisms . Here we show that the majority of Pmch-, Nfn- and Cart- expressing neurons were Dbx1-derived (Fig. 3B-D.iv). This finding combined with our previous loss-of-function studies is consistent with the hypothesis that Dbx1 acts cell autonomously to specify the fate of Pmch-, Nfn- and Cart- expressing neurons in the LH. Interestingly, the contribution of Dbx1-derived neurons to the Cart+ and Hcrt+ populations differed between males and females, revealing sexually dimorphic contributions of the Dbx1-lineage within the LH.
The other major neuronal output population of the LH is defined by expression of Hcrt, which, in contrast to the Pmch+ population, is activated in the presence of low glucose . Hcrt+ neuron specification, differentiation and number appear to require Lhx9 and histamine expression [37, 38]. Here, we reveal that the Hcrt-expressing population was at least partially Dbx1-derived, and also differed between males and females (Fig. 3E-E.iv).
In the Arc, three major neuronal populations are distinguished by their expression of Agrp, Pomc/Cart or tyrosine hydroxylase (TH) [39–41]. The expression of Bsx drives immature neurons towards a mature appetite-stimulating (orexigenic) Agrp+ fate, but not other Arc cell types such as the appetite-inhibiting (anorexigenic) Pomc neurons . Our previous studies in Dbx1 loss-of-function mice demonstrated an approximately 50 % reduction in Bsx and Agrp expression, with no changes in Pomc or TH expression . Our analysis here revealed that Dbx1-derived neurons contributed to all three populations, although at varying levels (Fig. 4). Dbx1-derived neurons contributed to 29–42 % of the Agrp+ population in males and females (Fig. 4B-B.iv). The Pomc-, TH- and Cart-expressing populations were 24–44 % Dbx1-derived, with the exception of the Pomc population in females, which was 52 % ± 6 % Dbx1-derived (mean ± SEM; Fig. 4C-E.iv). Of these Arc populations, the contribution of the Dbx1-derived lineage to the Pomc population was sexually dimorphic, with a greater contribution to Pomc+ neurons in females compared to males. Thus, the three major populations in the Arc are also derived from Dbx1-expressing progenitors.
Subsets of neuronal populations in the VMH can be defined by their expression of the sex steroid pathway markers Estrogen Receptor alpha (ERα) and Aromatase (Arom), which are known to function in mating and aggressive behaviors in mice [14, 43–45]. The majority of ERα neurons were Dbx1-derived (mean ± SEM; male: 66 % ± 7 %, female: 87 % ± 4 %; Fig. 5B-B.iv). In contrast, more variable results were observed with the Arom+ population, which depended on the sex of the animal. While the majority of the Arom+ population in females was Dbx1-derived, there was an apparently less, albeit more variable contribution of the Dbx1-lineage in males (male: 23 % ± 23 %, female: 78 % ± 14 %; Fig. 5C-C.iv). Overall, although varying by sex, these results show significant contribution of the Dbx1-lineage to the VMH ERα- and Arom-expressing populations.
Temporal labeling of Dbx1-derived neurons
Having established that different temporal waves of Dbx1-expressing progenitors occupy different post-natal medial to lateral hypothalamic domains, we next focused on development of subpopulations within the LH and Arc, two major nuclei with Dbx1-lineage contributions. Previous BrdU labeling studies in rats revealed that Pmch+ neurons are born in three major waves, with the early born neurons taking residence in the most lateral regions adjacent to the cerebral peduncle. Cells born later occupy progressively more medial portions of the tuberal mantle. The majority of Pmch+ neurons are born during mid stages thus occupying the majority of the zona incerta and LH regions surrounding the fornix . To investigate whether Dbx1-derived neurons develop in a similar pattern, we again used inducible fate-mapping and performed double immunohistochemistry. We observed proportionately more double-labeled neurons (Pmch+ and YFP+) at mid and later stages (TME9.5 and TME11.5; Fig. 6e-h). Thus, development of the Pmch+ Dbx1-derived population follows the same overall pattern of Pmch+ development, with the greatest contribution occurring between ~ E10.5-E13.0. Unlike the Pmch+ population, less is known about the timing of development of the Hcrt+ population. Here, we observed a marked increase in co-labeling of YFP and Hcrt at TME11.5, with little to no co-labeling at earlier stages (Fig. 6i-l). This suggests that the Dbx1-derived Hcrt+ population observed in Fig. 3E-E.iv most likely arises from a later wave of Dbx1 + progenitors.
Activation of Dbx1-derived neurons during innate behaviors
Different hypothalamic nuclei are engaged during processing of a variety of innate behaviors such as feeding, mating, aggression and predator odor avoidance [31, 50–54]. Specifically, c-Fos translation occurs in cells of the LH, Arc and VMH in response to fasting, predator odor, conspecific aggression and mating situations [19, 55–58]. Our previous studies revealed that Dbx1 functions in the specification of the LH and Arc neurons required for physiological and behavioral responses to innate stressors such as stress feeding and predator odor exposure . To investigate the putative involvement of Dbx1-derived neurons in different innate behaviors (predator odor, fasting, mating or aggression) we assessed the activation patterns of Dbx1-derived neurons in male and female Dbx1 Cre ; Rosa26 lox-STOP-lox-YFP mice using expression of the immediate early gene c-Fos as a proxy for neuronal activation [59, 60]. We focused our analyses on the LH, Arc and VMH, three major nuclei involved in these select innate behaviors.
The hypothalamus is a complex multi-nucleated structure in which individual nuclei function to direct a variety of behaviors essential for survival, adaptation and species propagation [2, 3]. Despite the extensive study of hypothalamic function and anatomy, only recently has there been a greater understanding of the mechanisms of hypothalamic development, predominantly via a combination of gene expression and lineage tracing studies. Our previous study focusing on the function of the embryonic expressed homeodomain encoding transcription factor, Dbx1 revealed Dbx1 to have a restricted function in the specification of hypothalamic neurons required for innate stress responses but not other innate behaviors . Here, we sought to extend these findings in order to provide deeper insight into the Dbx1-lineage contribution to diverse neuronal hypothalamic populations and to determine if Dbx1-derived neurons are activated by specific innate behaviors. We found a large amount of Dbx1-derived neuronal diversity across hypothalamic nuclei and broad activation of the Dbx1-lineage by different innate behaviors. Interestingly, the broad fate and generally non-selective activation of Dbx1-derived neurons to a variety of innate behaviors was not predicted by our previous finding of the restricted function of Dbx1 in specification of neurons solely required for innate stress responses. However, taken together these data are consistent with a model in which there are distinct gene sets expressed during hypothalamic development that, while maybe widespread in their lineage contribution, perform select functional roles in specification of distinct hypothalamic subpopulations.
Previous fate-mapping studies of the hypothalamus have begun to provide a general understanding of the relationship between progenitor domains and mature nuclei [3, 16, 18, 28, 61]. These studies have included examination of the lineage of a variety of developmentally defined subpopulations such as those that express the transcription factors Nkx2.1, Dlx, Nr5a1 [62, 63] and secreted factors such as Shh . Complementing this fate-mapping work are a series of detailed and highly informative hypothalamic developmental gene expression studies [27, 28, 64]. Collectively these studies have revealed that: 1) there are gene sets that give rise to neurons and function across hypothalamic nuclei (e.gs Rax, Nkx2.1, Asc1) and complementary gene sets that appear to be restricted in expression and function in specification of specific nuclei (e.gs Bsx, Nr5a1)  and 2) consistent with our findings, embryonic gene expression domains appear to be generally predictive of the location of mature nuclei, suggesting a general lack of widespread migration across domains. This is in contrast to the telencephalon where the ventral embryonic ganglionic eminence developmental domains (MGE and CGE) give rise to immature neurons that migrate to distant areas such as the cerebral cortex and hippocampus [65–67].
Within this framework, similar to progenitors expressing the developmentally regulated genes Shh and Nkx2.1, we found that Dbx1 + progenitors generate a wide variety of neuronal subtypes across multiple hypothalamic nuclei. Dbx1-derived cells were also present, although to a lesser degree, in the VMH. In contrast, regions of the anterior hypothalamus were devoid of Dbx1-derived neurons. A gradient of Dbx1-derived cells was also observed radiating from the tuberal domain into the anterior domain, a pattern that is more pronounced in medial portions of the ventral diencephalon. This pattern of Dbx1-derived neuronal location was generally shared with the pattern of location of Shh-lineage neurons . This finding perhaps reflects the overlapping embryonic expression domains of Shh and Dbx1 and may further indicate putative positive control of Dbx1 expression by Shh during forebrain development.
The hypothalamus has been thought to develop in an ‘outside-in’ manner, stemming from studies using traceable thymidine analogs indicating the lateral hypothalamic nuclei are typically born prior to medial nuclei [49, 68, 69]. More recent fate-mapping studies and analysis of molecularly defined cell types have provided another layer of complexity of development for select cell populations [48, 70]. Here, using inducible fate-mapping, we demonstrate that medial Dbx1-derived neurons in both LH and Arc are recombined earlier (before E9.5), with lateral populations recombined at later ages (after E9.5). Although our study is limited in that we did not conduct a birth-dating analysis of Dbx1-derived neurons, this observed pattern is consistent with previous Shh fate-mapping studies , and is supportive of a more complex pattern of medial-lateral development.
We further found Dbx1-derived neurons contribute to diverse molecularly defined populations in the LH, Arc and VMH. Within the LH, the majority of Pmch+ cells, which also express Nfn+, and Cart+, were Dbx1-derived. This finding is predicted by our loss-of-function studies in which the Pmch+ population was dramatically reduced . In the Arc, our Dbx1 loss-of-function studies demonstrated a ~50 % reduction of Agrp and Cart expression, with no changes in the Pomc+ or TH+ populations . Here we demonstrate that ~50 % of Agrp+ and Cart+ neurons were Dbx1-derived. Collectively these data are consistent with a cell autonomous function of Dbx1 in generation of LH Pmch+ and Arc Agrp+ populations. However, surprisingly, a significant proportion of Arc Pomc+ and TH+ cells were also Dbx1-derived. Thus, while Dbx1 + progenitors generate diverse populations in the Arc, it appears that Dbx1-independent mechanisms are required for specification of the Pomc+ and TH+ neurons.
At the behavioral level, the number of c-Fos+ cells in response to innate behavioral cues was increased in a predictable manner consistent with previous work [19, 55–57]. Building upon these results, we assessed the Dbx1-lineage contribution to these patterns of activation. We previously demonstrated that at the behavioral level conditional Dbx1 hypothalamic loss-of-function resulted in a specific defect in innate stress responses, but not other innate behaviors such as mating or aggression . Based on these findings, we anticipated that Dbx1-derived neurons would also be engaged (c-Fos+) selectively during innate stress behaviors (predator odor and fasting), but not other social behaviors (mating and aggression). In contrast, we found that across hypothalamic nuclei, Dbx1-derived neurons were active during multiple innate behavior tasks. Most broadly tuned to many behaviors was the LH, in which the percent of Dbx1-derived neurons expressing c-Fos increased after every behavioral paradigm tested. While this was not predicted by our previous loss-of-function studies, as we show here that a large portion the Pmch+ and Hcrt+ populations were Dbx1-derived, it is perhaps not surprising that the Dbx1-derived populations in the LH are responsive to a variety of innate cues.
These activation patterns, while still encompassing multiple behaviors, were more specific in the Arc and VMH. We observed an increase in the proportion of activated Dbx1-derived neurons after fasting, mating and male aggression in the Arc, and an increase after mating and male aggression in the VMH. In the Arc, while less than 50 % of the feeding neurons (Pomc, Agrp, and Cart) were Dbx1-derived, these neurons were c-Fos+ during fasting, likely reflecting their involvement in this major function of the Arc. In contrast, Arc Dbx1-derived neurons were less engaged in responses to predator odor. In the VMH, the Dbx1-derived neurons contributed to large portions of the ERα+ and Arom+ neuronal subpopulations, which are known to influence mating and aggressive behaviors [14, 43–45, 71]. This was reflected in the behavioral activation patterns, where the Dbx1-derived neurons were selectively activated during mating and aggression. While further experiments are needed to define the Dbx1-derived circuits that are required for specific hypothalamic-driven behaviors, these studies present novel insight into the link between developmental lineage and behavioral control.
In summary, using a combination of approaches we reveal a widespread and temporally regulated contribution of Dbx1 + progenitors to multiple neuronal populations across hypothalamic nuclei. We further demonstrate a broad innate behavioral tuning of Dbx1-derived cells in the LH, Arc and VMH, implicating their involvement in multiple innate behaviors. Thus, our studies provide new information regarding the link between hypothalamic embryonic gene expression patterns, postnatal neuronal fate, subtype identity and potential contribution to essential hypothalamic-driven behaviors.
We thank J. Triplett, and members of the Corbin and Triplett labs for constructive input during the course of this study. We thank A. Pierani for Dbx1 cre mice and S. Blackshaw, K. Campbell, C.M. Fan, P. Gray, K. Hashimoto-Torii, and Y. Nakagawa for ISH probes.
This work was partially supported by NIH grants, RO1NIDA020140, RO1NIDA020140S1, RO1NIDA020140S2 (J.G.C.) and F32DA035754 (K.S). S.E. was supported by the Uehara Memorial Foundation, Takeda science foundation and JSPS Institutional Program for Young Researcher Overseas Visits. Core support was received from the CNMC IDDRC Imaging Core (NIH IDDRC P30HD040677) and the CNMC Animal Neurobehavioral Core.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Gross CT, Canteras NS. The many paths to fear. Nat Rev Neurosci. 2012;13:651–8. doi:10.1038/nrn3301.View ArticlePubMedGoogle Scholar
- Sternson SM. Hypothalamic survival circuits: blueprints for purposive behaviors. Neuron. 2013;77:810–24. doi:10.1016/j.neuron.2013.02.018.View ArticlePubMedPubMed CentralGoogle Scholar
- Elson AE, Simerly RB. Developmental specification of metabolic circuitry. Front Neuroendocrinol. 2015. doi:10.1016/j.yfrne.2015.09.003.
- Caqueret A, Yang C, Duplan S, Boucher F, Michaud JL. Looking for trouble: a search for developmental defects of the hypothalamus. Horm Res. 2005;64:222–30.View ArticlePubMedGoogle Scholar
- Grossman SP. Role of the hypothalamus in the regulation of food and water intake. Psychol Rev. 1975;82:200–24.View ArticlePubMedGoogle Scholar
- O’Rahilly S. Human genetics illuminates the paths to metabolic disease. Nature. 2009;462:307–14. doi:10.1038/nature08532.View ArticlePubMedGoogle Scholar
- Morton GJ, Cummings DE, Baskin DG, Barsh GS, Schwartz MW. Central nervous system control of food intake and body weight. Nature. 2006;443:289–95.View ArticlePubMedGoogle Scholar
- Saper CB. Staying awake for dinner: hypothalamic integration of sleep, feeding, and circadian rhythms. Prog Brain Res. 2006;153:243–52.View ArticlePubMedGoogle Scholar
- Brown JA, Woodworth HL, Leinninger GM. To ingest or rest? Specialized roles of lateral hypothalamic area neurons in coordinating energy balance. Front Syst Neurosci. 2015;9:9. doi:10.3389/fnsys.2015.00009.PubMedPubMed CentralGoogle Scholar
- Saper CB, Lowell BB. The hypothalamus. Curr Biol. 2014;24:R1111–6. doi:10.1016/j.cub.2014.10.023.View ArticlePubMedGoogle Scholar
- Falkner AL, Lin D. Recent advances in understanding the role of the hypothalamic circuit during aggression. Front Syst Neurosci. 2014;8:168. doi:10.3389/fnsys.2014.00168.View ArticlePubMedPubMed CentralGoogle Scholar
- Burdakov D, Alexopoulos H. Metabolic state signalling through central hypocretin/orexin neurons. Eur J Neurosci. 2004;20:3281–5.View ArticlePubMedGoogle Scholar
- Xu X, Coats JK, Yang CF, Wang A, Ahmed OM, Alvarado M, Izumi T, Shah NM. Modular genetic control of sexually dimorphic behaviors. Cell. 2012;148:596–607. doi:10.1016/j.cell.2011.12.018.View ArticlePubMedPubMed CentralGoogle Scholar
- Lee H, Kim DW, Remedios R, Anthony TE, Chang A, Madisen L, Zeng H, Anderson DJ. Scalable control of mounting and attack by Esr1+ neurons in the ventromedial hypothalamus. Nature. 2014;509(7502):627–32. doi:10.1038/nature13169.View ArticlePubMedPubMed CentralGoogle Scholar
- Nomoto K, Lima SQ. Enhanced male-evoked responses in the ventromedial hypothalamus of sexually receptive female mice. Curr Biol. 2015;25:589–94. doi:10.1016/j.cub.2014.12.048.View ArticlePubMedGoogle Scholar
- Chatterjee M, Li JY. Patterning and compartment formation in the diencephalon. Front Neurosci. 2012;6:66. doi:10.3389/fnins.2012.00066.View ArticlePubMedPubMed CentralGoogle Scholar
- Sokolowski K, Corbin JG. Wired for behaviors: from development to function of innate limbic system circuitry. Front Mol Neurosci. 2012;5:55. doi:10.3389/fnmol.2012.00055.View ArticlePubMedPubMed CentralGoogle Scholar
- Bedont JL, Newman EA, Blackshaw S. Patterning, specification, and differentiation in the developing hypothalamus. Wiley Interdiscip Rev Dev Biol. 2015;4:445–68. doi:10.1002/wdev.187.View ArticlePubMedGoogle Scholar
- Sokolowski K, Esumi S, Hirata T, Kamal Y, Tran T, Lam A, Oboti L, Brighthaupt SC, Zaghlula M, Martinez J, Ghimbovschi S, Knoblach S, Pierani A, Tamamaki N, Shah NM, Jones KS, Corbin JG. Specification of select hypothalamic circuits and innate behaviors by the embryonic patterning gene Dbx1. Neuron. 2015;86:403–16. doi:10.1016/j.neuron.2015.03.022.View ArticlePubMedPubMed CentralGoogle Scholar
- Pierani A, Moran-Rivard L, Sunshine MJ, Littman DR, Goulding M, Jessell TM. Control of interneuron fate in the developing spinal cord by the progenitor homeodomain protein Dbx1. Neuron. 2001;29:367–84.View ArticlePubMedGoogle Scholar
- Hirata T, Li P, Lanuza GM, Cocas LA, Huntsman MM, Corbin JG. Identification of distinct telencephalic progenitor pools for neuronal diversity in the amygdala. Nat Neurosci. 2009;12:141–9. doi:10.1038/nn.2241.View ArticlePubMedPubMed CentralGoogle Scholar
- Vue TY, Aaker J, Taniguchi A, Kazemzadeh C, Skidmore JM, Martin DM, Martin JF, Treier M, Nakagawa Y. Characterization of progenitor domains in the developing mouse thalamus. J Comp Neurol. 2007;505(1):73–91.View ArticlePubMedGoogle Scholar
- Lu S, Bogarad LD, Murtha MT, Ruddle FH. Expression pattern of a murine homeobox gene, Dbx, displays extreme spatial restriction in embryonic forebrain and spinal cord. Proc Natl Acad Sci U S A. 1992;89:8053–7.View ArticlePubMedPubMed CentralGoogle Scholar
- Shoji H, Ito T, Wakamatsu Y, Hayasaka N, Ohsaki K, Oyanagi M, Kominami R, Kondoh H, Takahashi N. Regionalized expression of the Dbx family homeobox genes in the embryonic CNS of the mouse. Mech Dev. 1996;56:25–39.View ArticlePubMedGoogle Scholar
- Flames N, Pla R, Gelman DM, Rubenstein JL, Puelles L, Marín O. Delineation of multiple subpallial progenitor domains by the combinatorial expression of transcriptional codes. J Neurosci. 2007;27:9682–95.View ArticlePubMedGoogle Scholar
- Causeret F, Ensini M, Teissier A, Kessaris N, Richardson WD, Lucas de Couville T, Pierani A. Dbx1-expressing cells are necessary for the survival of the mammalian anterior neural and craniofacial structures. PLoS One. 2011;6:e19367. doi:10.1371/journal.pone.0019367.View ArticlePubMedPubMed CentralGoogle Scholar
- Kurrasch DM, Cheung CC, Lee FY, Tran PV, Hata K, Ingraham HA. The neonatal ventromedial hypothalamus transcriptome reveals novel markers with spatially distinct patterning. J Neurosci. 2007;27:13624–34.View ArticlePubMedGoogle Scholar
- Shimogori T, Lee DA, Miranda-Angulo A, Yang Y, Wang H, Jiang L, Yoshida AC, Kataoka A, Mashiko H, Avetisyan M, Qi L, Qian J, Blackshaw S. A genomic atlas of mouse hypothalamic development. Nat Neurosci. 2010;13:767–75. doi:10.1038/nn.2545.View ArticlePubMedPubMed CentralGoogle Scholar
- Verret L, Goutagny R, Fort P, Cagnon L, Salvert D, Léger L, Boissard R, Salin P, Peyron C, Luppi PH. A role of melanin-concentrating hormone producing neurons in the central regulation of paradoxical sleep. BMC Neurosci. 2003;4:19.
- Burdakov D, Luckman SM, Verkhratsky A. Glucose-sensing neurons of the hypothalamus. Philos Trans R Soc Lond B Biol Sci. 2005;360:2227–35.View ArticlePubMedPubMed CentralGoogle Scholar
- Maniam J, Morris MJ. The link between stress and feeding behaviour. Neuropharmacology. 2012;63:97–110. doi:10.1016/j.neuropharm.2012.04.017.View ArticlePubMedGoogle Scholar
- Yeo GS, Heisler LK. Unraveling the brain regulation of appetite: lessons from genetics. Nat Neurosci. 2012;15:1343–9. doi:10.1038/nn.3211.View ArticlePubMedGoogle Scholar
- Sohn JW, Elmquist JK, Williams KW. Neuronal circuits that regulate feeding behavior and metabolism. Trends Neurosci. 2013;36:504–12. doi:10.1016/j.tins.2013.05.003.View ArticlePubMedPubMed CentralGoogle Scholar
- Elias CF, Lee CE, Kelly JF, Ahima RS, Kuhar M, Saper CB, Elmquist JK. Characterization of CART neurons in the rat and human hypothalamus. J Comp Neurol. 2001;432:1–19.View ArticlePubMedGoogle Scholar
- Fort P, Salvert D, Hanriot L, Jego S, Shimizu H, Hashimoto K, Mori M, Luppi PH. The satiety molecule nesfatin-1 is co-expressed with melanin concentrating hormone in tuberal hypothalamic neurons of the rat. Neuroscience. 2008;155:174–81. doi:10.1016/j.neuroscience.2008.05.035.View ArticlePubMedGoogle Scholar
- Croizier S, Franchi-Bernard G, Colard C, Poncet F, La Roche A, Risold PY. A comparative analysis shows morphofunctional differences between the rat and mouse melanin-concentrating hormone systems. PLoS One. 2010;5:e15471. doi:10.1371/journal.pone.0015471.View ArticlePubMedPubMed CentralGoogle Scholar
- Sundvik M, Kudo H, Toivonen P, Rozov S, Chen YC, Panula P. The histaminergic system regulates wakefulness and orexin/hypocretin neuron development via histamine receptor H1 in zebrafish. FASEB J. 2011;25:4338–47. doi:10.1096/fj.11-188268.View ArticlePubMedGoogle Scholar
- Dalal J, Roh JH, Maloney SE, Akuffo A, Shah S, Yuan H, Wamsley B, Jones WB, Strong C, Gray PA, et al. Translational profiling of hypocretin neurons identifies candidate molecules for sleep regulation. Genes Dev. 2013;27:565–78. doi:10.1101/gad.207654.112.View ArticlePubMedPubMed CentralGoogle Scholar
- Chan-Palay V, Záborszky L, Köhler C, Goldstein M, Palay SL. Distribution of tyrosine-hydroxylase-immunoreactive neurons in the hypothalamus of rats. J Comp Neurol. 1984;227:467–96.View ArticlePubMedGoogle Scholar
- Broberger C. Hypothalamic cocaine- and amphetamine-regulated transcript (CART) neurons: histochemical relationship to thyrotropin-releasing hormone, melanin-concentrating hormone, orexin/hypocretin and neuropeptide Y. Brain Res. 1999;848:101–13.View ArticlePubMedGoogle Scholar
- Ovesjö ML, Gamstedt M, Collin M, Meister B. GABAergic nature of hypothalamic leptin target neurones in the ventromedial arcuate nucleus. J Neuroendocrinol. 2001;13:505–16.View ArticlePubMedGoogle Scholar
- Lee B, Kim SG, Kim J, Choi KY, Lee S, Lee SK, Lee JW. Brain-specific homeobox factor as a target selector for glucocorticoid receptor in energy balance. Mol Cell Biol. 2013;33:2650–8. doi:10.1128/MCB.00094-13.View ArticlePubMedPubMed CentralGoogle Scholar
- Sano K, Tsuda MC, Musatov S, Sakamoto T, Ogawa S. Differential effects of site-specific knockdown of estrogen receptor α in the medial amygdala, medial pre-optic area, and ventromedial nucleus of the hypothalamus on sexual and aggressive behavior of male mice. Eur J Neurosci. 2013;37:1308–19. doi:10.1111/ejn.12131.View ArticlePubMedGoogle Scholar
- Yang CF, Chiang MC, Gray DC, Prabhakaran M, Alvarado M, Juntti SA, Unger EK, Wells JA, Shah NM. Sexually dimorphic neurons in the ventromedial hypothalamus govern mating in both sexes and aggression in males. Cell. 2013;153:896–909. doi:10.1016/j.cell.2013.04.017.
- Dugger BN, Morris JA, Jordan CL, Breedlove SM. Androgen receptors are required for full masculinization of the ventromedial hypothalamus (VMH) in rats. Horm Behav. 2007;51:195–201.View ArticlePubMedPubMed CentralGoogle Scholar
- Danielian PS, Muccino D, Rowitch DH, Michael SK, McMahon AP. Modification of gene activity in mouse embryos in utero by a tamoxifen-inducible form of Cre recombinase. Curr Biol. 1998;8:1323–6.View ArticlePubMedGoogle Scholar
- Zervas M, Millet S, Ahn S, Joyner AL. Cell behaviors and genetic lineages of the mesencephalon and rhombomere 1. Neuron. 2004;43:345–57.View ArticlePubMedGoogle Scholar
- Alvarez-Bolado G, Paul FA, Blaess S. Sonic hedgehog lineage in the mouse hypothalamus: from progenitor domains to hypothalamic regions. Neural Dev. 2012;7:4.View ArticlePubMedPubMed CentralGoogle Scholar
- Risold PY, Croizier S, Legagneux K, Brischoux F, Fellmann D, Griffond B. The development of the MCH system. Peptides. 2009;30:1969–72. doi:10.1016/j.peptides.2009.07.016.View ArticlePubMedGoogle Scholar
- Yang S, Lee Y, Voogt JL. Fos expression in the female rat brain during the proestrous prolactin surge and following mating. Neuroendocrinology. 1999;69:281–9.View ArticlePubMedGoogle Scholar
- Atasoy D, Betley JN, Su HH, Sternson SM. Deconstruction of a neural circuit for hunger. Nature. 2012;488:17–177.View ArticleGoogle Scholar
- Betley JN, Cao ZF, Ritola KD, Sternson SM. Parallel, redundant circuit organization for homeostatic control of feeding behavior. Cell. 2013;155:1337–50. doi:10.1016/j.cell.2013.11.002.View ArticlePubMedPubMed CentralGoogle Scholar
- Cohen RS, Pfaff DW. Ventromedial hypothalamic neurons in the mediation of long-lasting effects of estrogen on lordosis behavior. Prog Neurobiol. 1992;38:423–53.View ArticlePubMedGoogle Scholar
- Silva BA, Mattucci C, Krzywkowski P, Murana E, Illarionova A, Grinevich V, Canteras NS, Ragozzino D, Gross CT. Independent hypothalamic circuits for social and predator fear. Nat Neurosci. 2013;16:1731–3. doi:10.1038/nn.3573.View ArticlePubMedPubMed CentralGoogle Scholar
- Lin D, Boyle MP, Dollar P, Lee H, Lein ES, Perona P, Anderson DJ. Functional identification of an aggression locus in the mouse hypothalamus. Nature. 2011;470:221–6. doi:10.1038/nature09736.View ArticlePubMedPubMed CentralGoogle Scholar
- Canteras NS, Chiavegatto S, Ribeiro do Valle LE, Swanson LW. Severe reduction of rat defensive behavior to a predator by discrete hypothalamic chemical lesions. Brain Res Bull. 1997;44:297–305.View ArticlePubMedGoogle Scholar
- Beijamini V, Guimarães FS. c-Fos expression increase in NADPH-diaphorase positive neurons after exposure to a live cat. Behav Brain Res. 2006;170:52–61.View ArticlePubMedGoogle Scholar
- García AP, Aitta-aho T, Schaaf L, Heeley N, Heuschmid L, Bai Y, Apergis-Schoute J. Nicotinic α4 receptor-mediated cholinergic influences on food intake and activity patterns in hypothalamic circuits. PLoS One. 2015;10(8):e0133327. doi:10.1371/journal.pone.0133327.
- Dragunow M, Faull R. The use of c-fos as a metabolic marker in neuronal pathway tracing. J Neurosci Methods. 1989;29:261–5.View ArticlePubMedGoogle Scholar
- Bullitt E. Expression of c-fos-like protein as a marker for neuronal activity following noxious stimulation in the rat. J Comp Neurol. 1990;296:517–30.View ArticlePubMedGoogle Scholar
- Hoch RV, Rubenstein JL, Pleasure S. Genes and signaling events that establish regional patterning of the mammalian forebrain. Semin Cell Dev Biol. 2009;20:378–86. doi:10.1016/j.semcdb.2009.02.005.View ArticlePubMedGoogle Scholar
- Yee CL, Wang Y, Anderson S, Ekker M, Rubenstein JL. Arcuate nucleus expression of NKX2.1 and DLX and lineages expressing these transcription factors in neuropeptide Y(+), proopiomelanocortin(+), and tyrosine hydroxylase(+) neurons in neonatal and adult mice. J Comp Neurol. 2009;517:37–50. doi:10.1002/cne.22132.View ArticlePubMedPubMed CentralGoogle Scholar
- Dhillon H, Zigman JM, Ye C, Lee CE, McGovern RA, Tang V, Kenny CD, Christiansen LM, White RD, Edelstein EA, Coppari R, Balthasar N, Cowley MA, Chua S Jr, Elmquist JK, Lowell BB. Leptin directly activates SF1 neurons in the VMH, and this action by leptin is required for normal body-weight homeostasis. Neuron. 2006;49:191–203.View ArticlePubMedGoogle Scholar
- Ferran JL, Puelles L, Rubenstein JL. Molecular codes defining rostrocaudal domains in the embryonic mouse hypothalamus. Front Neuroanat. 2015;9:46. doi:10.3389/fnana.2015.00046.View ArticlePubMedPubMed CentralGoogle Scholar
- Batista-Brito R, Fishell G. The developmental integration of cortical interneurons into a functional network. Curr Top Dev Biol. 2009;87:81–118. doi:10.1016/S0070-2153(09)01203-4.View ArticlePubMedPubMed CentralGoogle Scholar
- Nery S, Fishell G, Corbin JG. The caudal ganglionic eminence is a source of distinct cortical and subcortical cell populations. Nat Neurosci. 2002;5:1279–87.View ArticlePubMedGoogle Scholar
- Anderson SA, Marín O, Horn C, Jennings K, Rubenstein JL. Distinct cortical migrations from the medial and lateral ganglionic eminences. Development. 2001;128:353–63.PubMedGoogle Scholar
- Shimada M, Nakamura T. Time of neuron origin in mouse hypothalamic nuclei. Exp Neurol. 1973;41:163–73.View ArticlePubMedGoogle Scholar
- Markakis EA, Swanson LW. Spatiotemporal patterns of secretomotor neuron generation in the parvicellular neuroendocrine system. Brain Res Rev. 1997;24:255–91.View ArticlePubMedGoogle Scholar
- Padilla SL, Carmody JS, Zeltser LM. Pomc-expressing progenitors give rise to antagonistic neuronal populations in hypothalamic feeding circuits. Nat Med. 2010;16:403–5. doi:10.1038/nm.2126.View ArticlePubMedPubMed CentralGoogle Scholar
- Imwalle DB, Scordalakes EM, Rissman EF. Estrogen receptor alpha influences socially motivated behaviors. Horm Behav. 2002;42:484–91.View ArticlePubMedGoogle Scholar