Figure 3

Perinatal electroporation primarily targets proliferating cells. (A-A³) After pre-pulsing animals with BrdU 2 h prior to EP, most EGFP⁺ cells are BrdU⁺ as shown by the arrows. Arrowheads denote two EGFP⁺ cells with lower but detectable amounts of BrdU. Note that labeled cells with similar amounts of BrdU seem to occur as doublets, suggesting that they are daughter cells derived from the same mitotic event. Scale bars measure 100 μm in (A) and 20 μm in (A¹).