Rapid genetic targeting of pial surface neural progenitors and immature neurons by neonatal electroporation
- Joshua J Breunig†1Email author,
- David Gate†1, 2,
- Rachelle Levy1, 3,
- Javier RodriguezJr1,
- Gi Bum Kim1,
- Moise Danielpour3,
- Clive N Svendsen1, 2, 4 and
- Terrence Town1, 2, 3, 4Email author
© Breunig et al.; licensee BioMed Central Ltd. 2012
Received: 3 April 2012
Accepted: 25 June 2012
Published: 10 July 2012
Recent findings have indicated the presence of a progenitor domain at the marginal zone/layer 1 of the cerebral cortex, and it has been suggested that these progenitors have neurogenic and gliogenic potential. However, their contribution to the histogenesis of the cortex remains poorly understood due to difficulties associated with genetically manipulating these unique cells in a population-specific manner.
We have adapted the electroporation technique to target pial surface cells for rapid genetic manipulation at postnatal day 2. In vivo data show that most of these cells proliferate and progressively differentiate into both neuronal and glial subtypes. Furthermore, these cells localize to the superficial layers of the optic tectum and cerebral cortex prior to migration away from the surface.
We provide a foundation upon which future studies can begin to elucidate the molecular controls governing neural progenitor fate, migration, differentiation, and contribution to cortical and tectal histogenesis. Furthermore, specific genetic targeting of such neural progenitor populations will likely be of future clinical interest.
Beginning during embryogenesis and proceeding onward, the pattern of neurogenesis largely consists of neurons or neural progenitors born from asymmetric divisions of neuroepithelial cells along the neural tube (reviewed in ). Later on during early neurogenesis, these neuroepithelial cells convert to radial glia, but cell birth and migration generally continues to follow an inside-out pattern, with a few notable exceptions . In the human brain, for example, the earliest neurons appear to migrate tangentially from the subpallium . Another example is the tangential migration of rhombic lip progenitors to the external granule layer (EGL) of the cerebellum . In the EGL, these progenitors continue to divide before migrating inwards and subsequently differentiating into mature granule cells in the internal granule layer. Additionally, interneurons of the cerebral cortex and olfactory bulb also display a largely tangential mode of migration . In the case of cortical interneurons, these progenitors travel through several telencephalic compartments following their birth in the medial ganglionic eminence. Migrating interneurons can be found in the subventricular zone (SVZ) , the intermediate zone (IZ) , and the marginal zone (MZ) [7–9]. Notably, in the MZ, it has been shown that these interneurons arise from three distinct sources, seemingly displaying unique responses to local cues or responding to different sets of cues, as each population has different migration dynamics .
The marginal zone/layer 1 of the brain has recently become more appreciated as a site of neurogenesis and gliogenesis [10, 11]. Cell proliferation in this region occurs starting at the earliest time of neurogenesis , and even in the adult, neurogenesis has been reported to occur following hypoxia . Interestingly, this progenitor zone expands in both laminin γ1 knockout mice and Pax6 mutant mice . However, the above studies focused on disparate paradigms of embryonic neurogenesis and post-injury adult neurogenesis, and cell autonomous molecular investigation has been extremely difficult. Accordingly, the relative size and contribution of the postnatal marginal zone to histogenesis in the dorsal compartments of the brain remains under-explored. Specifically, it is not known whether interneurons are continually proliferating at the pial surface during the postnatal period. Moreover, the relative in vivo potential of this progenitor region remains unexplored due to lack of methods for facile genetic manipulation of these cells in a temporally and spatially constrained manner.
One such method that holds promise as a rapid delivery system for multiple genes simultaneously is electroporation (EP). EP is defined as the use of an electric current to drive charged macromolecules such as plasmid DNA into eukaryotic cells (reviewed in [12, 13]). EP of plasmids has been widely adopted as a tool for the rapid in vivo genetic manipulation of neural stem cells . Initially, it was used for in utero manipulation of radial glia surrounding the lateral ventricles of the mouse embryo . More recently, this technique has been extended for use in postnatal and adult brains [14–16]. However, except for one report in the hippocampus , most uses of EP to study neural stem cells involve injection of plasmid DNA into the brain ventricles.
We have adapted the EP technique to specifically and reproducibly target pial surface cells in multiple brain regions at postnatal day 2 (P2). Using this technique, we show that progenitors can be labeled and that they subsequently differentiate into neurons and astrocytes. This study builds a framework for future investigations aimed at studying the dynamics and molecular mechanisms of differentiation in this unique progenitor zone.
Electroporation of the dorsal surface of the cerebral cortex
To fine-tune the method, we attempted several different orientations of the tweezertrodes (Figure 1B, C). The first orientation directed current roughly parallel to the pial surface (though the surface is curved, so the EP vector is somewhat tangential). This allowed for successful EP of cells on the cortical surface (Figure 1D). Furthermore, we directed the current in a downward vector and observed a similar abundance of electroporated cells. (The parallel orientation was used for most experiments.) Electroporated cell counts with two different reporter plasmids (cytoplasmic EGFP and nuclear TagBFP) revealed signals that mostly colocalized (Figure 1D), and similar numbers of labeled cells (Figure 1E) were identified. Each label occupied the same general surface area (2.7 ± 0.64 mm2; n = 3). One concern might be that EP of radial glial end-feet occurred in lieu of pial surface cells, and that these cells may then convert into neural cells at the pial surface. However, we did not observe radial glial labeling two days post-EP using the present methodology (Figure 1F). In addition, when DNA solution was injected into the ventricle and electroporated with the same electrode orientation used for pial surface EP, our method did not label radial units associated with the medial cortex (Additional file 2: Figure S2A-A4). Instead, this approach resulted in labeling of radial glia that oriented: (1) inwards toward the hippocampus; or (2) towards the midline (Additional file 2: Figure S2A4).
Spatially confined electroporation of the tectum pial surface
Electroporated pial surface cells express neuronal and glial antigens
To more precisely define the electroporated cell type, we examined cells at 1 day post-EP. As mentioned, compared with 2 days post-EP, these younger electroporated cells were located near to or even at the pial surface (Figure 4A-A2). Two morphologies consistent with neural progenitors were frequently noted in labeled cells. The first was consistent with a glial progenitor phenotype. These cells often labeled with the astroglial marker Nfia, and were weakly positive (and sometimes negative) for Gfap (Figure 4B-B5; Figure 4C, denoted by an arrow). The other electroporated cell type was bipolar or lacked processes (Figure 4C-C4, denoted by arrowhead) and expressed the de facto interneuron transcription factor, MafB (Figure 4D-D4). This antigen labels ventral forebrain-derived interneuron progenitors and interneurons throughout their life cycle, starting in the SVZ of the medial ganglionic eminence in the forebrain .
Lineage assessment of pial surface electroporated cells
Differentiation of electroporated cells
Over the past decade, EP has become a powerful tool for genetic manipulation of neural stem and progenitor cells during embryonic and postnatal development [12, 23]. It has most often been utilized to target germinal zones surrounding the ventricles [13, 15, 16]. We have adapted and extended this technique to target the superficial layers of both the cortex and tectum at P2. In so doing, we provide a method for rapidly and specifically targeting these cell types. This technology will allow for elucidation of the genetic regulators of migration and differentiation of this population as well as their contribution to histogenesis during development and disease.
Our results demonstrate that postnatal EP can non-invasively target plasmids into the superficial progenitor domains rapidly and with a high degree of specificity. This methodology is given added importance, as we are not aware of any reporter mice or Cre recombinase lines capable of specifically targeting this unique pool of neural progenitors. Furthermore, while retroviruses have been used to specifically mark these cells , retroviral cloning and packaging is technically challenging and time-consuming. In addition, there are safety concerns associated with viral handling. EP simply requires purified plasmid DNA, which can be rapidly prepared. Furthermore, multiple genes or shRNAs can quickly be delivered, without the need for production of viral particles or complex mouse breeding strategies. However, it should be noted that the use of EP methodology and Cre-reporter or Cre-driver lines are not mutually exclusive.
We have provided a concise report of pial surface EP. The circumstantial evidence that we provide supports the progressive differentiation of immature neural progenitor populations into neurons and astroglia. Our results will need to be extended to rigorously determine the natural history of pial surface cell types and to exclude spurious or unexpected labeling mechanisms or transdifferentiation, which can be observed with such methods . Thus, we envision that the electroporation of plasmids with cell type-specific promoter elements driving Cre, or conversely, the delivery of Cre-dependent reporter plasmids into cell-lineage dependent Cre lines, will be invaluable for the definitive determination of cell potential and lineage commitment at the pial surface.
EP is most easily performed during the perinatal period, due to visibility of brain structures and the injection site through the skull. Furthermore, this technique most efficiently targets proliferating cells that are actively undergoing breakdown of the nuclear envelope, ostensibly allowing for episomal expression of the plasmid . Thus, during aging, EP efficiency would markedly decrease , as fewer and fewer cells are proliferating and targeting therefore becomes less accurate. Other potential caveats to EP include the episomal nature of most plasmids, whereby proliferation of cells may lead to plasmid dilution. Interestingly though, we did not observe much evidence of plasmid dilution within electroporated PS progenitors when compared to electroporated cells surrounding the lateral ventricles of the forebrain (data not shown). However, it did appear that astroglial EGFP expression declined between 14 days and 2 to 2.5 months post-EP, which is suggestive of further proliferation (data not shown). Nevertheless, these potential limitations could be overcome by using tools that allow for stable plasmid integration into the genome (for example, piggyBac as in , Tol2 , or Sleeping Beauty transposon systems).
Electroporation of the dorsal surface of the brain appears to target differentiating progenitor cells that initially do not express markers of mature neurons or astroglia. It is important to note that there are reports of in vivo postmitotic neuronal electroporation (reviewed in [23, 26–28]). However, by and large, these electroporation approaches are of the more invasive microelectroporation variety, where electrodes are inserted into the tissue vs. the external application used in this report. Notably, our BrdU labeling experiments did not label 100% of electroporated cells, and only weakly labeled some cells. However, the cell cycle lengthens throughout the course of embryogenesis  and throughout postnatal life , making BrdU labeling less efficient. In addition, there are likely temporal and regional differences in cell cycle length between the postnatal pial surface and cortical VZ, where previous EP/BrdU double-labeling experiments were carried out [17, 31]. Nevertheless, it is possible that we are targeting postmitotic populations, but these cells are likely to be immature, migrating, or otherwise in transition at the time of EP based on their progressive morphological differentiation. For example, EP of membrane-tagged EGFP demonstrated that the morphology of most cells was more undifferentiated, often in the form of multipolar glia or bipolar cells. At 14 days, cells displaying neuronal or astroglial morphologies did not strongly express the de facto markers of these respective cell types: NeuN (in both cortex and tectum) or Gfap (in the tectum only, as most non-activated cortical astrocytes do not normally express Gfap).
In our EP system, immunostaining and morphologic evidence of differentiated astroglia and neurons was present later on at 2 to 2.5 months. Even at this time, we did note that many cells with neuronal morphology did not label with NeuN. Notably, NeuN does not label all neuronal subtypes . In particular, it has been shown that some neurons in the piriform cortex are negative for this antigen . Based on available evidence then, we conclude that these cells represent a unique population of neurons that clearly display neuronal properties (thin dendrites stretching hundreds of μm, varicosities, and so on) and do not express non-neuronal lineage markers such as NG2, Olig2, or Aldolase C (data not shown). Interestingly, the longer time course of development of these unique pial progenitors seems to differ from typical VZ-derived neural cells. In summary, the perinatal EP technique detailed in this report should be amenable for rapid elucidation of the molecular mechanisms regulating this fascinating pial neural progenitor lineage.
In this report, we present a perinatal EP method for rapid genetic labeling and genetic modification of marginal zone/layer I progenitors in dorsal regions of the brain. This population has only recently begun to be characterized, and the cell autonomous genetic regulation of these unique neural progenitors remains completely undetermined. EP will likely prove to be a valuable tool for further exploration of these important neurobiological questions.
Subcloning and plasmid preparation
Briefly, the Ufek (U biquitin C promoter driving f arnesylated E GFP with K ozak site) plasmid was created by subcloning EGFP with a farnesylation domain from pEGFP-F (Clontech) into pUb-GFP (Plasmid 11155; Addgene) by In-fusion PCR subcloning. The pCagg-TagBFPnls construct was created by PCR subcloning the TagBFP coding sequence into pCAGEN with the inclusion of a V5 epitope tag and nuclear localization sequence in the carboxyl-terminal primer. Importantly, these plasmids lack nuclear import sequences associated with robust gene expression when transfected/electroporated into postmitotic cell types without transitioning through mitosis first . Endotoxin-free plasmids were maxi-prepped using Nucleobond Xtra Maxi EF kits (Macherey-Nagel). Additional cloning details are available upon request.
Postnatal day 2 mice were anesthetized by hypothermia. Injections were made free-hand using a pulled glass capillary pipette. During the injection, the capillary tube was used to penetrate the skin and skull. Using care, the tip was stopped from further penetration after first clearing the skull, which was indicated by the lack of further resistance felt just after piercing the skull. The left optic tectum was visually determined by locating lambda and then targeting the superior colliculus (just posterior and lateral to lambda) for injection. The skull was carefully pierced and 0.6 to 1.2 μL of plasmid solution containing fast green and 1.0 μg/μL of each plasmid was pressure-injected as in Figure 1A using a XenoWorks microinjection system. The cortex was similarly injected, but the injection was targeted to the center of the dorsal cortex as noted in Figure 2A. Care was taken to avoid the cerebral arteries, which might cause a local hematoma. Diagrams showing the injection strategy are shown in Additional file 1: Figure S1. Platinum tweezertrodes were then used to electroporate with three to five pulses of 135 V (50 ms; separated by 950 ms) generated using the ECM 830 BTX Electroporator (Harvard Apparatus). SignaGel was used to increase conductance. After EP, mice were placed on a heating pad prior to being returned to their cages. All animal experiments were performed in accordance with NIH guidelines, and were approved by the Cedars-Sinai Medical Center Institutional Animal Care and Use Committee under protocol IACUC003507.
In order to label dividing neural cells, pups were i.p. injected with 100 mg/kg of BrdU dissolved in sterile saline (10 mg/mL). This procedure was carried out 2 h prior to EP.
Electroporated pups were collected at defined postnatal ages, and their brains were immediately dissected in PBS and fixed in 4% PFA 6 h to overnight at 4 °C. On the following day, brains were embedded in 4% LMP agarose, sectioned at 70 or 250 μm thickness on a Leica VT1000S vibratome and then processed for immunohistochemistry.
Sections were reacted with various primary antibodies (diluted in PBS containing 10% normal donkey serum and 0.03% Triton-X) overnight at 4 °C. (For BrdU immunohistochemistry, tissue was treated with 2 N HCL for 15 min at 37 °C prior to immunostaining). Primary antibodies were detected using fluorescent-conjugated secondary antibodies (Jackson Immunobiology) diluted at 1:1,000 in PBS containing 0.03% Triton-X. The following primary antibodies were used: goat anti-Aldolase C (1:300; Santa Cruz), rat anti-BrdU (1:250; Accurate), goat anti-Olig2 (1:500; R&D Systems), goat anti-Dcx (1:500; Santa Cruz Biotechnology), mouse anti-Nestin (1:2,000; Abcam), chicken anti-EGFP (1:5,000; Abcam), guinea pig anti-Gfap (1:1,000; Synaptic Systems), rabbit anti-GluR1 (1:500; Millipore), rabbit anti-MafB (1:250-1,000; Bethyl Laboartories), mouse anti-NeuN (1:200; Millipore), rabbit anti-Nfia (1:1,000; active motif), rabbit anti-NG2 (1:250; Millipore), goat anti-Sox2 (1:500; Santa Cruz), mouse anti-Tuj1 (1:1,000; Sigma), rabbit anti-GFAP (1:2,000; Dako), and mouse anti-V5 (1:2,000; Life Technologies). All images were acquired on either a Zeiss Apotome, a Nikon A1R confocal, or a Nikon AZ-C1 macroconfocal microscope in independent fluorescence channels.
Electroporated cells were counted using ImageJ, release 1.45. The Nikon AZ-C1 macroconfocal was employed for quantification in Figures 1 and 2. All other quantification of electroporated populations was performed on images acquired with a Nikon A1R confocal microscope. Three images per region were acquired using a 20X objective and quantified using Nikon Elements, release 3.2. For 1 and 2 day quantifications, cells displaying apoptotic characteristics (pyknotic nuclei, membrane blebbing, detached processes) were disregarded. For lineage assessments, a total of 50 to 100 cells per region were quantified from 2-3 animals. Data represent averages of three sections from two to three animals.
Enhanced green fluorescent protein
External granule layer
Green fluorescent protein
In utero electroporation
Blue nuclear protein.
We thank the Margaret E. Early Medical Research Trust, the Cedars-Sinai Regenerative Medicine Institute, and the Department of Biomedical Sciences at Cedars-Sinai Medical Center for funding that helped to make this work possible. TT is supported by a National Institute of Health/National Institute on Neurologic Disorders and Stroke award (1R01NS076794-01), an Alzheimer’s Association Zenith Fellows Award (ZEN-10-174633), and an American Federation of Aging Research/Ellison Medical Foundation Julie Martin Mid-Career Award in Aging Research (M11472). TT is the inaugural holder of the Ben Winters Endowed Chair in Regenerative Medicine at Cedars-Sinai Medical Center.
- Breunig JJ, Haydar TF, Rakic P: Neural stem cells: historical perspective and future prospects. Neuron. 2011, 70: 614-625. 10.1016/j.neuron.2011.05.005.PubMed CentralView ArticlePubMedGoogle Scholar
- Bystron I, Rakic P, Molnar Z, Blakemore C: The first neurons of the human cerebral cortex. Nat Neurosci. 2006, 9: 880-886. 10.1038/nn1726.View ArticlePubMedGoogle Scholar
- Alder J, Cho NK, Hatten ME: Embryonic precursor cells from the rhombic lip are specified to a cerebellar granule neuron identity. Neuron. 1996, 17: 389-399. 10.1016/S0896-6273(00)80172-5.View ArticlePubMedGoogle Scholar
- Marin O, Rubenstein JL: Cell migration in the forebrain. Annu Rev Neurosci. 2003, 26: 441-483. 10.1146/annurev.neuro.26.041002.131058.View ArticlePubMedGoogle Scholar
- Anderson SA, Marin O, Horn C, Jennings K, Rubenstein JL: Distinct cortical migrations from the medial and lateral ganglionic eminences. Development. 2001, 128: 353-363.PubMedGoogle Scholar
- Tamamaki N, Fujimori KE, Takauji R: Origin and route of tangentially migrating neurons in the developing neocortical intermediate zone. J Neurosci. 1997, 17: 8313-8323.PubMedGoogle Scholar
- Ang ES, Haydar TF, Gluncic V, Rakic P: Four-dimensional migratory coordinates of GABAergic interneurons in the developing mouse cortex. J Neurosci. 2003, 23: 5805-5815.PubMedGoogle Scholar
- Lavdas AA, Grigoriou M, Pachnis V, Parnavelas JG: The medial ganglionic eminence gives rise to a population of early neurons in the developing cerebral cortex. J Neurosci. 1999, 19: 7881-7888.PubMedGoogle Scholar
- Zecevic N, Rakic P: Development of layer I neurons in the primate cerebral cortex. J Neurosci. 2001, 21: 5607-5619.PubMedGoogle Scholar
- Costa MR, Kessaris N, Richardson WD, Gotz M, Hedin-Pereira C: The marginal zone/layer I as a novel niche for neurogenesis and gliogenesis in developing cerebral cortex. J Neurosci. 2007, 27: 11376-11388. 10.1523/JNEUROSCI.2418-07.2007.View ArticlePubMedGoogle Scholar
- Ohira K, Furuta T, Hioki H, Nakamura KC, Kuramoto E, Tanaka Y, Funatsu N, Shimizu K, Oishi T, Hayashi M, Miyakawa T, Kaneko T, Nakamura S: Ischemia-induced neurogenesis of neocortical layer 1 progenitor cells. Nat Neurosci. 2010, 13: 173-179. 10.1038/nn.2473.View ArticlePubMedGoogle Scholar
- Breunig JJ, Arellano JI, Macklis JD, Rakic P: Everything that glitters isn't gold: a critical review of postnatal neural precursor analyses. Cell Stem Cell. 2007, 1: 612-627. 10.1016/j.stem.2007.11.008.View ArticlePubMedGoogle Scholar
- Saito T, Nakatsuji N: Efficient gene transfer into the embryonic mouse brain using in vivo electroporation. Dev Biol. 2001, 240: 237-246. 10.1006/dbio.2001.0439.View ArticlePubMedGoogle Scholar
- Barnabe-Heider F, Meletis K, Eriksson M, Bergmann O, Sabelstrom H, Harvey MA, Mikkers H, Frisen J: Genetic manipulation of adult mouse neurogenic niches by in vivo electroporation. Nat Methods. 2008, 5: 189-196. 10.1038/nmeth.1174.View ArticlePubMedGoogle Scholar
- Boutin C, Diestel S, Desoeuvre A, Tiveron MC, Cremer H: Efficient in vivo electroporation of the postnatal rodent forebrain. PLoS One. 2008, 3: e1883-10.1371/journal.pone.0001883.PubMed CentralView ArticlePubMedGoogle Scholar
- Chesler AT, Le Pichon CE, Brann JH, Araneda RC, Zou DJ, Firestein S: Selective gene expression by postnatal electroporation during olfactory interneuron nurogenesis. PLoS One. 2008, 3: e1517-10.1371/journal.pone.0001517.PubMed CentralView ArticlePubMedGoogle Scholar
- Stancik EK, Navarro-Quiroga I, Sellke R, Haydar TF: Heterogeneity in ventricular zone neural precursors contributes to neuronal fate diversity in the postnatal neocortex. J Neurosci. 2010, 30: 7028-7036. 10.1523/JNEUROSCI.6131-09.2010.PubMed CentralView ArticlePubMedGoogle Scholar
- Cobos I, Long JE, Thwin MT, Rubenstein JL: Cellular patterns of transcription factor expression in developing cortical interneurons. Cereb Cortex. 2006, 1 (Suppl): 82-i88.View ArticleGoogle Scholar
- Marshall CA, Novitch BG, Goldman JE: Olig2 directs astrocyte and oligodendrocyte formation in postnatal subventricular zone cells. J Neurosci. 2005, 25: 7289-7298. 10.1523/JNEUROSCI.1924-05.2005.View ArticlePubMedGoogle Scholar
- Mullen RJ, Buck CR, Smith AM: NeuN, a neuronal specific nuclear protein in vertebrates. Development. 1992, 116: 201-211.PubMedGoogle Scholar
- Klempin F, Kronenberg G, Cheung G, Kettenmann H, Kempermann G: Properties of doublecortin-(DCX)-expressing cells in the piriform cortex compared to the neurogenic dentate gyrus of adult mice. PLoS One. 2011, 6: e25760-10.1371/journal.pone.0025760.PubMed CentralView ArticlePubMedGoogle Scholar
- Nacher J, Alonso-Llosa G, Rosell D, McEwen B: PSA-NCAM expression in the piriform cortex of the adult rat. Modulation by NMDA receptor antagonist administration. Brain Res. 2002, 927: 111-121. 10.1016/S0006-8993(01)03241-3.View ArticlePubMedGoogle Scholar
- De Vry J, Martinez-Martinez P, Losen M, Temel Y, Steckler T, Steinbusch HW, De Baets MH, Prickaerts J: In vivo electroporation of the central nervous system: a non-viral approach for targeted gene delivery. Prog Neurobiol. 2010, 92: 227-244. 10.1016/j.pneurobio.2010.10.001.View ArticlePubMedGoogle Scholar
- Chen F, Loturco J: A method for stable transgenesis of radial glia lineage in rat neocortex by piggyBac mediated transposition. J Neurosci Methods. 2012, 207: 172-180. 10.1016/j.jneumeth.2012.03.016.PubMed CentralView ArticlePubMedGoogle Scholar
- Yoshida A, Yamaguchi Y, Nonomura K, Kawakami K, Takahashi Y, Miura M: Simultaneous expression of different transgenes in neurons and glia by combining in utero electroporation with the Tol2 transposon-mediated gene transfer system. Genes Cells. 2010, 15: 501-512.PubMed CentralPubMedGoogle Scholar
- Judkewitz B, Rizzi M, Kitamura K, Hausser M: Targeted single-cell electroporation of mammalian neurons in vivo. Nat Protoc. 2009, 4: 862-869. 10.1038/nprot.2009.56.View ArticlePubMedGoogle Scholar
- Kitamura K, Judkewitz B, Kano M, Denk W, Hausser M: Targeted patch-clamp recordings and single-cell electroporation of unlabeled neurons in vivo. Nat Methods. 2008, 5: 61-67. 10.1038/nmeth1150.View ArticlePubMedGoogle Scholar
- Wei F, Xia XM, Tang J, Ao H, Ko S, Liauw J, Qiu CS, Zhuo M: Calmodulin regulates synaptic plasticity in the anterior cingulate cortex and behavioral responses: a microelectroporation study in adult rodents. J Neurosci. 2003, 23: 8402-8409.PubMedGoogle Scholar
- Takahashi T, Nowakowski RS, Caviness VS: The cell cycle of the pseudostratified ventricular epithelium of the embryonic murine cerebral wall. J Neurosci. 1995, 15: 6046-6057.PubMedGoogle Scholar
- Hayes NL, Nowakowski RS: Dynamics of cell proliferation in the adult dentate gyrus of two inbred strains of mice. Brain Res Dev Brain Res. 2002, 134: 77-85.View ArticlePubMedGoogle Scholar
- Cai L, Hayes NL, Nowakowski RS: Local homogeneity of cell cycle length in developing mouse cortex. J Neurosci. 1997, 17: 2079-2087.PubMedGoogle Scholar
- Dean DA, Dean BS, Muller S, Smith LC: Sequence requirements for plasmid nuclear import. Exp Cell Res. 1999, 253: 713-722. 10.1006/excr.1999.4716.PubMed CentralView ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.