Figure 10

UNC-9 and UNC-7 fail to heteromerize. (A) Co-expression in interneurons of UNC-7S (red) and UNC-9::GFP (Punc-7S::unc-9::gfp; green) in unc-7(e5) shows little co-localization (yellow). (For clarity DAPI staining is not shown; positions of B motor neuron nuclei are indicated.) UNC-7S does not concentrate in puncta near B cell bodies. (B) Pcex-1::unc-7S::gfp (AVA, AVD interneurons) rescues forward locomotion but does not concentrate in puncta near B cell bodies. (C) Co-expression of Pcex-1::unc-7S (red) and Pcex-1::unc-9::gfp (green) shows little co-localization in an unc-7(e5) background. (D) UNC-7L expressed in interneurons (Punc-7S::unc-7L [M121L]) rescues forward locomotion in unc-7(e5) and localizes to puncta near B motor neuron cell bodies. (E) UNC-7L expressed in B motor neurons (Pacr-5::unc-7L [M121L]) in unc-9 daf-6 unc-7 does not localize to puncta but remains associated with cell bodies (red); co-expressed UNC-9::GFP (Pacr-5::unc-9::gfp; green) is distributed throughout the motor neuron processes.