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Fig. 6 | Neural Development

Fig. 6

From: Follicle-innervating Aδ-low threshold mechanoreceptive neurons form receptive fields through homotypic competition

Fig. 6

Aδ-LTMRs adjust their receptive field size to accommodate changes in neuronal population due to Bax deletion. A-C’) Representative images and traced reconstructions of isolated Aδ-LTMR receptive fields from back skin of P21 TrkBCreERT2; R26iAP; Bax wild-type (A, A’), heterozygous (B, B’), and knockout (C, C’) animals. Red dots in traces correspond with hair follicles, green stars indicate the axon segment from which the traced RF originates. Scale bar – 250 μm. D Quantification of the number of hair follicles innervated by each Aδ-LTMR receptive field in TrkBCreeRT2;R26iAP; Bax wild-type, heterozygous, and knockout animals. Receptive fields in Bax knockout animals innervate fewer hair follicles than wild-type and heterozygous animals. Each dot represents one receptive field, red dots represent the data point from to the associated representative image. Bars represent mean ± s.e.m. One-way AONVA with Tukey’s HSD post-hoc testing ANOVA P ≤ 0.001, WT-KO P ≤ 0.001, Het-KO P ≤ 0.001, WT-Het P = 0.043. Number of individual receptive fields (number of animals): WT: 85(3), Het: 154(5), KO: 103(3). EG’ Representative images and traced reconstructions of isolated C-LTMR receptive fields from back skin of P21 THCreERT2; R26iAP;Bax wild-type (E, E’), heterozygous (F, F’), and knockout (G, G’) animals. Red dots in traces correspond with hair follicles, green stars indicate the axon segment from which the traced RF originates. Scale bar – 250 μm. H Quantification of the number of hair follicles innervated by each C-LTMR receptive field in THCreERT2; R26.iAP;Bax wild-type, heterozygous, and knockout animals. No significant difference was seen in the number of hair follicles innervated across Bax genotypes. Each dot represents one receptive field, the red dots represent the data point from to the associated representative image. Bars represent mean ± s.e.m. One-way AONVA, P = 0.098. Number of individual receptive fields (number of animals): WT: 90(6), Het: 22(4), KO: 51(3)

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