Fig. 5
Genetic deletion of the proapoptotic protein Bax has differential effects on LTMR populations. A-F) Representative sections of T6 dorsal root ganglia from 21-day old wild-type littermates (A-C) and Bax knockout (D-F) mice labeled with markers for neuronal nuclei (A, D Islet 1/2), Aδ-LTMR cell bodies (B, E TrkBCreERT2; R26iAP), C-LTMR cell bodies (C, F Tyrosine Hydroxylase). Scale bar – 50 μm. Insets show high-magnification of boxed regions. G Quantification of the change in T6 DRG LTMR neuron proportions in Bax knockout animals normalized to the mean number of heterozygous littermates. Bars represent mean ± s.e.m. N = Islet 1/2: Het – 7, KO – 5, TrkB: Het – 5, KO – 3, TH: Het – 7, KO – 5. Islet 1/2 Wilcoxon Rank Sum: P ≤ 0.040, TrkB.CreERT2 Wilcoxon Rank Sum: P ≤ 0.007, TH Wilcoxon Rank Sum: P > 0.089