Fig. 3
Aβ RA-LTMR neurons complete innervation of hair follicles before C-LTMR neurons. A-F Representative images of hair follicle innervation labeled with a Aβ RA-LTMR specific marker (Calbindin, green) and DAPI (magenta). Mature longitudinal-lanceolate endings begin to appear by P3. Arrowheads (A, E) – Subcutaneous muscle autofluorescence, Scale bar – 50 μm. G Quantification of the percentage of hair follicles innervated by a Calbindin positive Aβ RA-LTMR axon. The percentage of innervated hair follicles reaches its maximum level by P3. One-way ANOVA with Tukey’s HSD post-hoc testing (ANOVA P ≤ 0.001, P1-P3 P ≤ 0.001). N = 6(P1), 3(P3), 4(P6), 5(P14), 3(P21), 4(P60). H-M) Representative images of hair follicles innervated by Vglut3 + axons (Vglut3iCre;TdTomato, green) and DAPI (magenta). Mature longitudinal-lanceolate endings begin to appear by P3. Arrowhead (M) – Sebaceous gland autofluorescence, Scale bar – 50 μm. N Quantification of the percentage of hair follicles innervated by a Vglut3.iCre;TdTomato positive C-LTMR axon. The percentage of innervated hair follicles increases between P1 and P3, and reaches its maximum level by P6. One-way ANOVA with Tukey’s HSD post-hoc testing (ANOVA P ≤ 0.001, P1-P3 P ≤ 0.001, P3-P6 P ≤ 0.001). N = 3(P1), 3(P3), 3(P6), 5(P14), 3(P21), 3(P60)