Fig. 5
From: Primary neurons lacking the SNAREs vti1a and vti1b show altered neuronal development
Neurotrophic factors did not induce neurite growth in DKO. Hippocampal neurons were isolated from E15.5 and E18.5 DHET or DKO embryos, cultivated for 24 h and treated with 100 ng/mL NGF (E18.5, a), 25 ng/mL BDNF (E18.5, b), or 50 ng/mL GDNF (E18.5, d) for 24 h or treated directly after preparation with 30 ng/mL NT-3 (E15.5, c) for 48 h. Neurite outgrowth was quantified by measuring the longest neurite per neuron and by calculation the average length for each embryo. At least 100 cells were measured per time point. Number of embryos: NGF: 3 DHET, 3 DKO; BDNF: 7 DHET, 9 DKO; NT-3: 3 DHET, 4 DKO; GDNF: 6 DHET, 6 DKO. The bars represent the mean ± SEM, *: p < 0.05, ***: p < 0.001 two-way ANOVA