Fig. 1

Deviating somatic distribution of Golgi in DKO neurons. Hippocampal neurons were isolated at E18.5 and cultivated for 8 days in vitro (8 DIV). (a) DHET and DKO neurons were identified with anti-MAP2 antibodies (white) and the Golgi apparatus were labeled with anti-GM130 antibodies (red). The white line was added in ImageJ to mark the cell body. Confocal Z-stacks were taken and maximum intensity projections (MIP) were presented. (b) Stacks of pictures were taken and the pictures with the largest Golgi area were quantified (GM130 pixels above threshold within the cell body divided by area of the cell body). (c) DKO neurons were transfected with GFP-Vti1a at 3 DIV and were further cultivated for 5 DIV. Cells were stained and (d) analyzed as described above. N = 3 embryos with > 15 cells per experiment ± SEM ***: P < 0.001, P > 0.05 not significant (ns) unpaired student’s t-test, scale bars (a) and (c) 20 μm