Fig. 6

m6A and Ythdf increase target protein abundance in neuroblasts and neurons. A Representative images of Runt in neuroblasts (outlined by white dotted line) and neurons (cells clustered below neuroblast) in wildtype, Mettl3 null and Ythdf overexpressing brains. The fluorescent signal intensity (mean and standard deviation) for Runt in each genotype and cell type is shown at right. B Representative images of CycD in neuroblasts (outlined by white dotted line) in wildtype, Mettl3 null and Ythdf overexpressing brains. CycD was not detected in neurons. The fluorescent signal intensity (mean and standard deviation) for CycD in each genotype is shown at right. C Representative images of Ase in neuroblasts (outlined by white dotted line) in wildtype, Mettl3 null and Ythdf overexpressing brains. Ase was not detected in neurons. The fluorescent signal intensity (mean and standard deviation) for Ase in each genotype is shown at right. All fluorescent intensity measurements are derived from analysis of ≥ 20 cells from ≥ 6 different brain lobes. Statistical significance was determined by one way ANOVA followed by Tukey post-test. P-values: ** = 1 × 10–4, *** ≤ 1 × 10–7