Fig. 5
From: Dpr10 and Nocte are required for Drosophila motor axon pathfinding
nocte is broadly expressed in larvae. (A-D) Larval VNCs from nocte-GAL4 x UAS-NLS-GFP animals stained for GFP (green) and cell specific markers (magenta). (A′-D‴) Insets of A-D (cyan rectangles) showing co-localization of GFP and the cell specific markers. (E-E″) VNC of nocte-GAL4 x UAS-G-TRACE larva stained for GFP (green) indicating only early expression (arrow) and RFP (magenta) indicating only late expression (caret). Cell expressing both GFP and RFP (arrowhead) suggest nocte is constitutively expressed. (F-G) nocte-GAL4 x UAS-NLS-GFP animals stained for GFP (green) and neuronal tissue (HRP, blue). (F-F″) Peripheral nerves stained for the glial marker Repo (magenta). (F′-F″) Insets of cyan rectangle in F. Note that only a subset of glial cells express nocte. (G) Ventral muscle field showing expression in m13, 12, 5, 4 but not m6 (nuclei lacking GFP outlined in dotted ellipses). All scale bars = 50 μm