Fig. 2
From: Dpr10 and Nocte are required for Drosophila motor axon pathfinding
Immunoprecipitation followed by Mass Spectrometry of Dpr10 uncovers in vivo interactors. (A) Larval brain of Dpr10-V5 expressed with dpr10-GAL4. Preps were stained for V5 (green) to visualize Dpr10 and HRP (magenta) to visualize neurons. (B) Larval body wall of Dpr10-V5 expressed in muscles (Mef2-GAL4). Scale bars = 100 μm. (C) Unique peptides recovered from immunoprecipitation of Dpr10-V5 and analyzed by mass spectrometry from brain samples (left, plum) and muscles (right, gray). Note that Nocte is the only associated protein recovered from both samples. Control immunoprecipitations did not have peptides for any of these proteins