Mature neuron conclusion. A An atlas of mature neurons (Brp and nSyb positive) was made with a subset of 51,596 cells from the whole atlas. B UMAP of atlas from 1h-48h alh with clusters 4 and 5 outlined. (C) Validated cell identity markers label distinct neuronal cell types. D Dot plot of differentially expressed factors between cluster 4 and 5. E Dot plot of top differentially* expressed genes between 1h alh, 24h alh and 48h alh in labeled mature neurons. F Dot plot of differentially* expressed cell surface molecules between 1h alh and 24h alh in labeled mature neurons. 48h alh contained no differentially expressed cell surface molecule genes. G Dot plot of differentially* expressed transcription factors between 1h alh, 24h alh and 48h alh in labeled mature neurons. *Genes found differentially expressed in at least 2 out of 9 annotated clusters of differentiated neuron cell types.