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Fig. 2 | Neural Development

Fig. 2

From: Single cell RNA-seq analysis reveals temporally-regulated and quiescence-regulated gene expression in Drosophila larval neuroblasts

Fig. 2

Quiescent neuroblasts and glial cells show enriched markers for regulating the TOR and insulin pathway. A UMAP of CNS cell types with quiescent neuroblasts in cluster 8 (circled). B UMAP of cluster from 1h-48h ALH. C Dot plot of top cluster defining genes alongside validated cell identity markers for quiescent neuroblasts. D Dot plot of genes involved with cell cycle regulation including the insulin signaling, AKT and TOR pathways. E UMAP re-clustered of 11,004 glia cells from a subset of the whole atlas. Diagram adapted from Kremer et al, 2017 [73]. F Validated glial cell type markers. G Temporal expression of signaling molecules involved in neuroblast quiescence within glial subtypes. H Validation of InR and Foxo expression in Dpn+ quiescent neuroblasts. Scale bar, 5 uM. I Model depicting cell growth and cell cycle genes identified as significantly enriched or depleted in the quiescent neuroblast cluster at 1h ALH, placed in the context of known signaling pathways

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Neural Development

ISSN: 1749-8104