Fig. 1

Larval atlas shows distinct cell identities and differentiating neural progenitor axis. A An atlas of 97,845 cells collected from 1h, 24h and 48h ALH larvae was built. These cells were analyzed with Seurat and clustered to identify major cell types such as neural progenitors, glial, mature neurons and other features to validate the atlas in clustering by cell type. B Feature plots of Dpn and Hey show a differentiating neural progenitor axis. The mature neuronal marker nSyb shows limited expression in progenitors that extends into mature neuronal clusters. The glial marker repo shows glial cells separated from progenitors and mature neurons. C Validated cell identity markers label distinct progenitor cell types within a developmental axis. D Progenitor atlas was made with a subset from the whole atlas of 33,458 neural progenitor and immature neuron cells. Black line indicates expected developmental trajectory. E UMAPs of progenitor clusters from 1h-48h ALH