Fig. 2

Expression of POU4F3 and ATOH1 in the early developing cochlea of the common marmoset. (A) In E77, no expression of POU4F3 and ATOH1 was detected in the cochlear, while expression of POU4F3 or ATOH1 was detected in the vestibular hair cells (arrow in A). (B-D) In E87, expression of POU4F3 and ATOH1 was detected in the basal and mid-basal turn. No expression of POU4F3 or ATOH1 was detected in the apical turn. In the basal turn, expression of POU4F3 and ATOH1 was detected in the inner hair cells and one row of the outer hair cells, while in the mid-basal turn, their expression was observed only in the inner hair cells. (E–G) In E92, POU4F3 and ATOH1 expression was observed in the middle and basal turns. No expression of POU4F3 or ATOH1 was detected in the apical turns. POU4F3 and ATOH1 expression was observed in both the inner and outer hair cells in both the middle and basal turns. Nuclei were counterstained with Hoechst stain (blue). Scale bar: 200 µm in A, 50 µm in B-G. VE, vestibular organ; CO, cochlea; IHC, inner hair cells; OHC, outer hair cells