Fig. 12

Early development and integration of the glial cells of the cochlear spiral ganglion. (A and B) Glial cells in the E70 cochleovestibular ganglion. SOX10⁺/S100B⁺ glial cells were observed in the E70 cochleovestibular ganglion (A). Notably, the small number of SOX10⁻/SOX2⁺/S100B⁺ glial cells were also observed at this stage (arrowhead in B). (C-F) Glial cells in the E77 spiral ganglion. At E77, glial cells were observed surrounding the spiral ganglion neurons at this stage (arrow in E). No SOX10-positive cells were observed in the center of the spiral ganglion (asterisk in C). At this stage, S100B-positive glial cells expressed both SOX2 and SOX10 (F). (G-I) Glial cells in E87. Several SOX10-positive cells were observed between the spiral ganglion neurons (arrow in G). (J-L) Glial cells in E92. Integration of the SOX10-positive glial cells in the spiral ganglion was more prominent. The number of SOX10-positive cells observed between the spiral ganglion neurons increased (arrow in J). The nuclei were counterstained with Hoechst (blue). Scale bar: 20 µm in A, B, D, and F. 50 µm in C, E, and G-L