Fig. 4

Differential expression of DSCAM by RGC axon fibers in retina. a Epifluorescent image of a coronal section of a stage 45 tadpole eye immunostained with DSCAM (red; right panel DSCAM only) and 3A10 antibodies (green) shows strong DSCAM immunoreactivity in the in the ganglion cell layer (GCL), inner plexiform layer (IPL) and Inner nuclear layer (INL). 3A10 immunopositive cell bodies are confined to the GCL, adjacent to the IPL. b Frontal and orthogonal confocal views of an eye in a cleared Xenopus tadpole head immunostained with DSCAM (red) and 3A10 anti-neurofilament (green) antibodies shows strong DSCAM immunoreactivity in the GCL and IPL and differential distribution of 3A10 immunoreactivity in a subset of cells in the GCL (lines indicate location of x–z and y–z orthogonal planes, thickness of sample imaged was 296 µm). c Confocal images of a tadpole eye illustrate DSCAM and 3A10 immunoreactivity at the level of the optic fiber layer (arrow) within the retina and in the optic nerve head (box). d The magnified images (box in c) show a subset of 3A10-immunopositive axon fibers (white arrowheads) that do not express DSCAM (left panel, 3A10 and DSCAM overlap; right panel, DSCAM only). e, f A single confocal plane shows coincident immunoreactivity for DSCAM and 3A10 (empty arrowheads) in ventral fibers along the optic nerve head, as also illustrated in d (empty arrowhead). Scale bars are as shown for each image