Fig. 5

Both nrp2a and nrp2b act in the same pathway with sema3fa. A Representative confocal sections of sema3fa mutant and nrp2a;sema3fa double mutant siblings. Yellow arrows indicate misprojecting axons. B The misprojection phenotype in nrp2a;sema3fa double mutants is not significantly different from sema3fa mutant siblings. C Representative confocal sections of sema3fa mutant and nrp2b;sema3fa double mutant siblings. Yellow arrows indicate misprojecting axons. D The misprojection phenotype in nrp2b;sema3fa double mutants is not significantly different from sema3fa mutant siblings. Model of average TRPC2-class axon locations in sema3fa heterozygotes and mutants during live imaging sequence. TRPC2-class axons are shown in green and magenta, and OMP-class axons in grey. The three TRCP2 surfaces encompass axon location probabilities, from most transparent to most opaque, of 5.6, 18.7, and 31.8%. Yellow dotted line represents the edge of the dorsal-medial OB region