Fig. 4

Corto and Psc genetically interact to close the early competence window. A i), NB7-1 lineage early competence assay comparing wild type, corto^L1/+, Psc^h27/+, or corto ^L1/+;Psc^27/+ transheterozygous embryos. Bars represent mean ± SD. ii), Scatterplot of wild type and corto ^L1/+;Psc^27/+ data, showing the number of Eve⁺HA⁺ (early-born) neurons relative to the total number of Eve neurons within an individual NB7-1 lineage. Each data point represents competence data of a single NB7-1 lineage quantified from three to four embryos per genotype. B Schematic circular diagram depicting results. C Early competence window test with and without co-overexpression of Corto. Example NB7-1 lineage shown for Hb and Corto co-misexpressed by en-Gal4 driver, stained for Hb^HA (cyan) and Eve (red). Quantification shown to the right. Each data point represents the average of at least twelve neuroblast lineages from a single embryo. Bars represent mean ± SD