Fig. 3

Temporal dynamics of Hb repression in neuroblasts is not affected in corto mutants. A Ventral view of VNC wild type or corto mutant (corto^L1) embryos at late stage 12, when the last few neuroblasts in the thoracic segments still express Hb (cyan), but the rest, including all abdominal neuroblasts, are no longer Hb⁺. Neuroblasts are marked by pan-neuroblast marker, Dpn (red). B At stage 13, Hb is no longer detectable in any neuroblasts (top panels) but can be detected in neurons, deeper in the VNC (bottom panels). Hb shown alone in grayscale in A and B. C Quantification of fraction of neuroblasts expressing Hb at stage 11 and stage 12 comparing wild type and corto mutant embryos, showing similar decrease in Hb neuroblast expression over time. D Schematic diagram summarizing results