Fig. 5
From: A subpopulation of astrocyte progenitors defined by Sonic hedgehog signaling
Shh signaling is upregulated in mature astrocytes (A-H) Double labeling for tdTom (A, E, red) and βGal (B, grey, F, green) in the cortex of a Gli1CreER/nLacZ;Ai14 mouse marked at P0 and analyzed at P60. (A-D) Low magnification epifluorescent and (E-H) high magnification confocal images. Counterstained with DAPI (C, G, blue). Dotted line depicts white matter border. Merged images shown in D, H. bv, blood vessel. Arrowheads, colocalized cells; arrows, βGal only cells. I The fraction of tdTom or βGal labeled cells that are single labeled (n = 1454 and n = 821 cells, respectively, from 3 animals). Bars represent mean ± SEM, data points represent individual animals