Fig. 2
From: A subpopulation of astrocyte progenitors defined by Sonic hedgehog signaling
Cells expressing Gli1 at P0 generate cortical astrocytes. A-D Cells marked at P0 and analyzed at P1 (A), P3 (B), P7 (C), and P14 (D) show an increasingly complex morphology consistent with an astrocyte identity as development proceeds. E Single cell quantification of the fraction of marked cells that correspond to astrocytes or oligodendrocytes. Data points represent individual animals (n = 2–4 animals). At least 150 cells/animal analyzed. F-M Single channel (F-H, J-L) and merged images (I, M) from confocal images of tdTom (F, J, red) and S100β (G, green) or CC1 (K, green) in the cortex at P14, following tamoxifen administration at P0. Marked cells show colocalization with S100β, but not with CC1. Counterstaining with DAPI (H, L, blue). Arrowheads, colocalized cells; arrows, single labeled astrocytes or oligodendrocytes not marked with tdTom. Scale bar, 25 um