Fig. 1
From: A subpopulation of astrocyte progenitors defined by Sonic hedgehog signaling
The population of Gli1 cells expands during postnatal development. A-D The distribution of βGal-labeled cells in Gli1nlacZ/+ tissues at P0. (A) Neurolucida tracing from brightfield immunostained sections. Each dot represents a single cell. B-C Brightfield (B) and immunofluroescent (C) staining of βGal at P0 in the cortex (B) and SVZ (C). Insets in C shown in C1 and C2. Scale bars, B, C1-C2, 50 μm, C, 100 μm. lv, lateral ventricle, vSVZ, ventral SVZ, dlSVZ, dorsolateral SVZ. D The number of βGal-labeled cells in the cortex and dlSVZ at P0. Bars represent mean ± SEM, data points represent individual animals (n = 6). Statistical significance assessed by unpaired Student’s t-test. E Schematic depicting experimental timeline. F-I TdTom (red) expression in the cortex of Gli1CreER/+;Ai14 mice that received tamoxifen at P0 and analyzed at P1 (F), P3 (G), P7 (H), and P14 (I) shows a broad distribution of marked cells throughout the cortex that expands over time. Counterstaining with DAPI (blue), wm, white matter. Scale bar, 50 μm. J The fraction of marked cells (Gli1) and dividing cells (BrdU) that are double labeled in mice marked at P0 and analyzd at P14 (n = 919 Gli1 and n = 820 BrdU cells, from 2 animals). Bars represent mean ± SEM, data points represent individual animals. K Immunofluorescent staining for BrdU in the cortex of a mouse at P14 that received tamoxifen at P0. Single channel and merged max projection images from confocal stacks in the cortex showing colocalization of marked cells (red) and BrdU (green). Counterstaining with DAPI (blue). Arrowheads, double labeled cells. Scale bar, 25 μm